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pDONR221-SLC7A5_STOP

Plasmid

#161363

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A5 (SLC7A5 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-Apex2-Venus-CAAX

Plasmid

#168511

Purpose

Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

Depositor

Franck Polleux

Article

Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

Insert

Apex2

Tags

Venus-CAAX

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM5-mSHMT2

Plasmid

#83905

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 2 (Shmt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Nkx6.2

Plasmid

#64846

Purpose

A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx6.2 (Nkx6-2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-Tubulin-C-18

Plasmid

#56176

Purpose

Localization: Microtubules, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tubulin (TUBA1B Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
SOX2 TALE Repressor (KRAB 11-75)

Plasmid

#42946

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

SOX2 TALE Repressor (KRAB 11-75)

Use

Lentiviral

Tags

2A-GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pIS1-Vim5UTR-renilla

Plasmid

#38237

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim 5'UTR / Renilla (Vim Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Vim 5' UTR fused to renilla ORF

Promoter

Endogenous Vim

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pBS_hNFATc4nuc

Plasmid

#24586

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

NFATc4 (NFATC4 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S167A, S169A, S172A, S173A, S174A, S213A, S217A, …

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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Nkx6.2

Plasmid

#15542

Depositor

John Rubenstein

Article

Qiu et al Mech Dev. 1998 Mar . 72(1-2):77-88.

Insert

Nkx6.2 (Nkx6-2 Mouse)

Expression

Bacterial

Mutation

Partial cDNA

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Myc-His(-)-MSulf-1

Plasmid

#13007

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

mouse sulfatase 1 (Sulf1 Mouse)

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pMax_mPlum

Plasmid

#177830

Purpose

Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)

Viral Prep

#198516-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Promoter

EF-1a

Tags

mScarlet

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only
IVT-donor_opt(R2Tg)-GFP

Plasmid

#223246

Purpose

In vitro transcription of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

In vitro transcription

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_G299C

Plasmid

#177829

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_G299C

Expression

Mammalian

Mutation

G299C

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-mCherry

Plasmid

#49647

Purpose

TALERed-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

mCherry

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF3412

Plasmid

#144888

Purpose

Lentiviral vector for overexpressing the EGR1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EGR1 (EGR1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-SURF4

Plasmid

#170680

Purpose

Expresses FLAG-tagged SURF4 in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

SURF4

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_Ubiquitin

Plasmid

#131258

Purpose

Mammalian expression of ubiquitin with an N-terminal HA tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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