We narrowed to 64,639 results for: cell;

1. pRK5 DN-Fyn

   Plasmid

   #16033

   Depositor

   Filippo Giancotti

   Article

   Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

   Insert

   c-Fyn (FYN Human)
   

   Expression

   Mammalian

   Mutation

   Dominant negative: K299M

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

   Plasmid

   #215328

   Purpose

   Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

   Depositor

   Rob Hynds

   Article

   Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

   Insert

   FOXJ1 Promoter
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   FOXJ1

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA-mKeima-PIM

   Plasmid

   #174650

   Purpose

   Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

   Depositor

   Lukas Kapitein

   Article

   Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

   Insert

   xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
   

   Expression

   Mammalian

   Mutation

   Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

   Promoter

   Chicken beta-actin

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

   Plasmid

   #69534

   Purpose

   DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   DD-dCas9VP192-T2A-EGFP
   

   Tags

   C-term T2A-EGFP and DHFR Destabilised Domain

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

   Plasmid

   #62956

   Purpose

   Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-Cremyc-2A-GFP

   Plasmid

   #116879

   Purpose

   Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

   Depositor

   Qiang Lu

   Article

   Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

   Insert

   Cremyc-2A-GFP
   

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5HT6-YFP-PIPK(D253A)

   Plasmid

   #96811

   Purpose

   kinase-dead control for 5HT6-YFP-PIPK

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-PIPK(D253A)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D253A inactivates kinase activity of PIPK

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

   Plasmid

   #191154

   Purpose

   Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   EGFP-t70587
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ETV4_pet28a

   Plasmid

   #131645

   Purpose

   Bacterial expression of ETS gene ETV4

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV4 (ETV4 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABEPuro-HA-GLI1

    Plasmid

    #62967

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pHRi_1G4 hTCRβ-SNAP

    Plasmid

    #187604

    Purpose

    Lentiviral expression of hTCR beta(1G4), and SNAP for low expression

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    1G4 hTCR beta, and SNAP tag (TRB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mHSP

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-T7-NlaCas3-NLS-6xHis

    Plasmid

    #180214

    Purpose

    Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas3c
    

    Tags

    6xHis and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ Zf GFP-vclb

    Plasmid

    #185411

    Purpose

    GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin b (vclb Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MDH1-PGK-H2Kb2

    Plasmid

    #17852

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    H-2Kb (H2-K1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-KS-attB2-SA(0)-T2A-Gal4

    Plasmid

    #62899

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-KDM4D

    Plasmid

    #188234

    Purpose

    Express human KDM4D in mammalian cells

    Depositor

    Young Tang

    Article

    Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

    Insert

    Lysine demethylase 4D (KDM4D Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

    Plasmid

    #62954

    Purpose

    Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ Zf GFP-vcla

    Plasmid

    #185410

    Purpose

    GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin a (vcla Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

    Plasmid

    #62955

    Purpose

    Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-eGFP-NMHC-IIAΔtailpiece

    Plasmid

    #35689

    Depositor

    Tom Egelhoff

    Article

    Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

    Insert

    MHC IIA (MYH9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Added stop codon after codon 1928, eliminating th…

    Promoter

    CMV

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only