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pAAV-Ef1a-FAS-EGFP-WPRE-pA

Plasmid

#37091

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

d1eGFP

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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PITX3-eGFP-PGK-Puro

Plasmid

#31942

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP

Plasmid

#21731

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-Venus

Plasmid

#19966

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)

Tags

Flag and monomeric Venus

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pAMPK gamma1

Plasmid

#15996

Depositor

Morris Birnbaum

Insert

AMPK gamma1 (Prkag1 Mouse)

Tags

His6

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
F-MED26

Plasmid

#15367

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED26 (MED26 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pTK222

Plasmid

#13532

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Tags

See map

Expression

Mammalian

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pRK Smad3 deltaC Flag

Plasmid

#12626

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated Smad3 (aa 1-382)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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TU#710

Plasmid

#15278

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pBS hIhh (CT#402)

Plasmid

#13995

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Ihh (IHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Plasmid

#163055

Purpose

Grx1-roGFP2 glutathione redox sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSVK3-Flag-MAN1

Plasmid

#26002

Depositor

Howard Worman

Article

Wu et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1361-7

Insert

LEMD3 LEM domain containing 3 (LEMD3 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
CF-PIPKi

Plasmid

#20158

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-PIPK (D253A) (Pip5k1c Mouse)

Expression

Mammalian

Mutation

D253A mutation in PIPK. C-terminus (amino acids …

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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EMSV-myogenin (puro)

Plasmid

#14712

Depositor

Michael Rudnicki

Article

Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

Insert

myogenin (Myog Rat)

Expression

Mammalian

Available Since

April 2, 2007

Availability

Academic Institutions and Nonprofits only

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LRP6 deltaN-pCS2-VSVG

Plasmid

#27281

Depositor

Xi He

Article

Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

VSVG

Mutation

contains amino acid residues 1370–1613 (extracell…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-D77

Plasmid

#18896

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-D77 (UBC Human)

Expression

Bacterial

Mutation

Aspartate residue is added to the C-terminus of t…

Available Since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IL1B

Plasmid

#67066

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL1B (IL1B Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGP-AAV-syn-iGABASnFR2-WPRE (AAV5)

Viral Prep

#218874-AAV5

Purpose

Ready-to-use AAV5 particles produced from pGP-AAV-syn-iGABASnFR2-WPRE (#218874). In addition to the viral particles, you will also receive purified pGP-AAV-syn-iGABASnFR2-WPRE plasmid DNA. Syn-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Promoter

Syn

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only