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AKARdual

Plasmid

#99869

Purpose

Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

AKAR4

Tags

LSSmOrange and mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VPR

Plasmid

#183936

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

Plasmid

#115207

Purpose

Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

BzoCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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SPDK3860

Plasmid

#199245

Purpose

Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Promoter

pPEBV

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR tdTomato L5-L2

Plasmid

#62185

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xMS2_SL]

Plasmid

#68426

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three MS2 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ10333

Plasmid

#183955

Purpose

CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

Nuclease-dead Lb Cas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta

Plasmid

#32453

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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YFP-SspBx6

Plasmid

#103778

Purpose

expression of iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-SspBx6

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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C413-E45: attB4-hr5-ie1p>-attB1r

Plasmid

#162930

Purpose

Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV ie1 promoter with hr5 enhancer

Use

Synthetic Biology

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
ePPEmax*

Plasmid

#205244

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG-FFLuc-3XFLAG

Plasmid

#127333

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CV40

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Tags

N-myristoylation

Expression

Insect

Promoter

GAL4-responsive UAS hsp70

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3xSNAP-NLS

Plasmid

#236388

Purpose

Overexpression of PCP-3xSNAP in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

PCP-3xSNAP

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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PDCD1-DEM (P1#5)

Plasmid

#227738

Purpose

Designer Epigenome Modifier (DEM) designed to target the PDCD1-promoter

Depositor

Claudio Mussolino

Article

Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

Insert

Designer epigenome modifier targeting PDCD1-promoter

Use

TALEN

Tags

DNMT3A, DNMT3L, KRAB

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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LAG3-DEM (L3#1)

Plasmid

#227739

Purpose

Designer Epigenome Modifier (DEM) designed to target the LAG3-promoter

Depositor

Claudio Mussolino

Article

Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

Insert

Designer epigenome modifier targeting LAG3-promoter (Homo sapiens)

Use

TALEN

Tags

DNMT3A, DNMT3L, KRAB

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sapphireCKAR(T/A)

Plasmid

#118469

Purpose

Negative-control mutant for sapphireCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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