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pcDNA6-N-Flag-SREBP2

Plasmid

#134284

Purpose

Expresses Flag-tagged SREBP2 in mammalian cells

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Publication

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Expression

Bacterial

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Publication

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 blast

Plasmid

#104997

Purpose

This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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FLAG-EglN3-pLenti6

Plasmid

#36951

Depositor

William Kaelin

Publication

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN3 (EGLN3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GST HP1 gamma

Plasmid

#24076

Depositor

Naoko Tanese

Publication

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
plRL117

Plasmid

#163635

Purpose

Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Jacquere (split-vector)

Pooled library

#247026

Purpose

Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

Depositor

John Doench, David Root

Publication

Drepanos et al Cell Genom. 2026 May 13;6(5):101190. doi

Has service

Lentiviral Prep

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only
HSPB1_pEGFP-C1-2µ-URA3

Plasmid

#251717

Purpose

Expresses human HSPB1 tagged with EGFP in mammalian cells

Depositor

Svetlana Konovalova

Insert

HSPB1 (HSPB1 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR del52-linker-Venus-KDEL

Plasmid

#214786

Purpose

Mammalian expression of human del52-linker-Venus-KDEL

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR del52-linker-Venus-KDEL (CALR Human)

Use

Retroviral

Promoter

MSCV

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR ins5-linker-Venus-KDEL

Plasmid

#214787

Purpose

Mammalian expression of human CALR ins5-linker-Venus-KDEL

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR ins5-linker-Venus-KDEL (CALR Human)

Use

Retroviral

Promoter

MSCV

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-N Terminus (pGEX4T1)

Plasmid

#238422

Purpose

Expresses GRP78 N terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-C73 (pGEX4T1)

Plasmid

#238428

Purpose

Expresses GRP78 with the only the last 73 amino acids at the C terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C17 (pGEX4T1)

Plasmid

#238426

Purpose

Expresses GRP78 with the last 17 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C11(pGEX4T1)

Plasmid

#238425

Purpose

Expresses GRP78 with the last 11 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C73 (pGEX4T1)

Plasmid

#238427

Purpose

Expresses GRP78 with the last 73 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta KDEL (pGEX4T1)

Plasmid

#238424

Purpose

Expresses GRP78 Delta KDEL fused to the GST Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-C Terminus (pGEX4T1)

Plasmid

#238423

Purpose

Expresses GRP78 C Terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-No UTRs AAA Mutant (pcDNA3.1)

Plasmid

#238430

Purpose

Expresses GRP78 AAA mutant without the UTRs fused to FLAG tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The UTRs of the gene have been deleted. The PPP t…

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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