We narrowed to 265,412 results for: ins

1. pKK-TEV-mCerulean

   Plasmid

   #105790

   Purpose

   Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mCerulean

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-mCardinal-TEV

   Plasmid

   #105781

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mCardinal-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-mCerulean-TEV

   Plasmid

   #105773

   Purpose

   Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mCerulean-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

   Plasmid

   #79030

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   deleted amino acid 294-515 from frican green monk…

   Promoter

   polyhedrin

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. KC1G3_HUMAN_D0

   Plasmid

   #79685

   Purpose

   This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KC1G3 (CSNK1G3 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pQCXIX_sh_hMIB2_#9

   Plasmid

   #75255

   Purpose

   retroviral shRNA vector against human MIB2, expresses GFP for transduction control

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   shRNA against MIB2 (human) (MIB2 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZS1[GalS-L,RbsR-L]

   Plasmid

   #60763

   Purpose

   Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   GalS-L
   RbsR-L
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. LacI DBD with GalS L…

   Available Since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZS1[GalS-L,FruR-L]

   Plasmid

   #60764

   Purpose

   Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   GalS-L
   FruR-L
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. LacI DBD with FruR L…

   Available Since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZS1[GalS-L,TreR-L]

   Plasmid

   #60765

   Purpose

   Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   GalS-L
   TreR-L
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. LacI DBD with GalS L…

   Available Since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZS1[FruR-L,RbsR-L]

    Plasmid

    #60761

    Purpose

    Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    FruR-L
    RbsR-L
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with FruR L…

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZS1[RbsR-L,TreR-L]

    Plasmid

    #60766

    Purpose

    Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    RbsR-L
    TreR-L
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with RbsR L…

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid

    #111731

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q145 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

    Plasmid

    #111730

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q109 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

    Plasmid

    #111744

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q30 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

    Plasmid

    #111727

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q54 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

    Plasmid

    #111746

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q42 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36

    Plasmid

    #111745

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q36 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60

    Plasmid

    #111749

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q60 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23

    Plasmid

    #111726

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19

    Plasmid

    #111733

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q19 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge