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C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-Puro-ARHGAP26

Plasmid

#69467

Purpose

Expresses ARHGAP26

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

ARHGAP26 (ARHGAP26 Human)

Use

Retroviral

Tags

HA TAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro-SNX2-PRDM6

Plasmid

#69470

Purpose

Expresses SNX2-PRDM6 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

SNX2-PRDM6 (SNX2, PRDM6 Human)

Use

Retroviral

Tags

HA TAG

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV MN (eGFP)

Plasmid

#89932

Purpose

Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

MN

Tags

HA

Expression

Mammalian

Mutation

M.SssI residues 2-272 fragment

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLNCX Myr His p110 KD

Plasmid

#10837

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 KD (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Mutation

D915S. Kinase Dead.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pRS306:VN

Plasmid

#37555

Depositor

Wei-Lih Lee

Article

Markus et al Cytoskeleton (Hoboken). 2011 Mar;68(3):1

Insert

Venus N-term (aa 1-272)

Expression

Bacterial

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pLNCX Myr His p110

Plasmid

#10836

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-Puro-CLDN18-ARHGAP26

Plasmid

#69465

Purpose

Expresses Cldn18-Arhgap26 fusion gene.

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

CLDN18-ARHGAP26 (ARHGAP26, CLDN18 Human)

Use

Retroviral

Tags

HA TAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro- MLL3-PRKAG2

Plasmid

#69473

Purpose

Expresses MLL3-PRKAG2 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

MLL3-PRKAG2 (PRKAG2 Human)

Use

Retroviral

Tags

HA TAG:

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro-CLEC16A-EMP2

Plasmid

#69468

Purpose

Expresses CLEC16A-EMP2 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

CLEC16A-EMP2 (CLEC16A, EMP2 Human)

Use

Retroviral

Tags

HA TAG

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Cherry-Plekhh1 5 silent mutations

Plasmid

#187373

Purpose

Expresses human Plekhh1 with 5 silent mutations labelled with Cherry

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1 with 5 silent mutations

Tags

mCherry

Expression

Mammalian

Mutation

Five silent mutations at the Plekhh1 siRNA site (…

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST14-ARL4D

Plasmid

#67384

Purpose

Expression of native hArl4D in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N215-273C

Plasmid

#17527

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 216-272 (homeodomain)

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCMV sMTase (eGFP)

Plasmid

#89930

Purpose

Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC-IRES-MN

Tags

Flag and HA

Expression

Mammalian

Mutation

deactivated Cas9

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL4D

Plasmid

#67336

Purpose

hArl4D with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4D-no stop

Plasmid

#67337

Purpose

hArl4D without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
Tpa1(273-644)

Plasmid

#23141

Depositor

SeWon Suh

Article

Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

Insert

Tpa1 C-terminal domain (TPA1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-272

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCMV MN (eGFP)

Plasmid

#89932

Purpose

Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

MN

Tags

HA

Expression

Mammalian

Mutation

M.SssI residues 2-272 fragment

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-mPU.1-ETS

Plasmid

#85733

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

Depositor

Gregory Poon

Article

Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

Insert

ETS domain of transcription factor PU.1 (Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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