We narrowed to 9,186 results for: mel

1. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

   Plasmid

   #52419

   Purpose

   Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   ERAS (ERAS Human)
   

   Promoter

   CAGGS

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

   Plasmid

   #216733

   Purpose

   Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase and sgCTG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX551-miniCMV-SpCas9D10A nickase

   Plasmid

   #216735

   Purpose

   Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   T7 and mini-CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER20 MEF2C S222A

   Plasmid

   #89718

   Purpose

   Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MEF2C (MEF2C Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed serine 222 to alanine

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

   Plasmid

   #171801

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMEM230-M4/X184PG-IRES(isoform 2)

   Plasmid

   #99568

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M4/X184PG

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TMEM230-M3/Y92C-IRES(isoform 2)

   Plasmid

   #99567

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M3/Y92C

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TMEM230-M2/X184W-IRES(isoform 2)

   Plasmid

   #99566

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M2/X184W

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TMEM230-M1/R141L-IRES(isoform 2)

   Plasmid

   #99565

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M1/R141L

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-IRES-Tomato MEF2C S222A

    Plasmid

    #89716

    Purpose

    Retroviral gene expression vector for MEF2C-S222A expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid

    #171813

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-IRES_Tomato MEF2C S222D

    Plasmid

    #107231

    Purpose

    hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S222D

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TMEM230-M2/X184W-IRES(isoform 1)

    Plasmid

    #99560

    Purpose

    mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Mutation

    M2/X184W

    Promoter

    CMV

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TMEM230-M1/R141L-IRES(isoform 1)

    Plasmid

    #99559

    Purpose

    mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Mutation

    M1/R141L

    Promoter

    CMV

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-spCas9

    Plasmid

    #231404

    Purpose

    Expresses spCas9 from hSyn promoter

    Depositor

    Arjen Boender, Larry Young

    Article

    Schimmer et al bioRxiv 2024.07.15.603543; doi: https://

    Insert

    hSYN promoter (RIC8B Human)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    hSYN

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB OROV Gc Spike H6

    Plasmid

    #229662

    Purpose

    Make PiggyBac stable cell line expressing Oropouche virus BeAn 19991 Gc spike region (residues 482–894 of M polyprotein) with C-terminal His6 tag

    Depositor

    Stephen Graham

    Article

    Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

    Insert

    OROV Gc spike
    

    Use

    Piggybac

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    contains residues 482-894 only

    Promoter

    TRE

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid

    #202554

    Purpose

    Synaptotagmin-1 sgRNA1 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pInducer 21-3xFlag-hMEFV-ΔPLD

    Plasmid

    #195479

    Purpose

    pInducer21 plasmid containing the human MEFV gene with a deletion of residues 88 - 310 (the resulting pyrin protein lacks the Phosphorylated Linker Domain domain) with a 3xFlag tag at the N-terminus

    Depositor

    Thomas Henry

    Article

    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d

    Insert

    MEFV (MEFV Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    deletion of residues 88 - 310 (the resulting pyri…

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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