We narrowed to 8,728 results for: pcDNA3

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mc2 puro hPGK

Plasmid

#206227

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 neo hPGK

Plasmid

#206226

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro EF1a

Plasmid

#206222

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro

Plasmid

#206216

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pB2AR-miRFP718nano

Plasmid

#195751

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

B2AR-miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP718nano

Plasmid

#195748

Purpose

LC-Myosin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-LC-Myosin

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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VPS13D(∆ATG2-C/PH)^EGFP

Plasmid

#194004

Purpose

Deletion of DHPH domain of VPS13D

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D (VPS13D Human)

Tags

EGFP

Expression

Mammalian

Mutation

early stop codon before DHPH

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP718nano

Plasmid

#195752

Purpose

G protein alpha-subunit internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

G protein alpha-s-miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-INS-sg

Plasmid

#194719

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (INS Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo

Plasmid

#191398

Purpose

HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-NLS-MaSat ZF-18-mVenusN

Plasmid

#191392

Purpose

Zinc finger fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

Flag-NLS-MaSat ZF-18-mVenusN

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2369-Tier1-PhCMV_KRAB

Plasmid

#169571

Purpose

Tier-1 vector encoding PhCMV-driven KRAB transsilencer domain (PhCMV-KRAB-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Kruppel associated box domain

Expression

Mammalian

Promoter

PhCMV

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pVH228-Tier1-OVanO2-PCMVmin2-SEAP

Plasmid

#169564

Purpose

Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP production

Expression

Mammalian

Promoter

VanO2-PCMVmin

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX11-Tier1-PhCMV-Clover

Plasmid

#169508

Purpose

Tier-1 vector encoding PhCMV-driven gren fluorescent protein clover (PhCMV-clover-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven green fluorescent protein clover

Expression

Mammalian

Promoter

PhCMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2370-Tier1-PhCMV-TetR_F-type

Plasmid

#169573

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVH254-Tier1-OTetO7-Pmin-SEAP

Plasmid

#169585

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-Pmin-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

Plasmid

#169588

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-2

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2373-Tier1-PhCMV-Gal4

Plasmid

#169581

Purpose

Tier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

regulatory protein GAL4

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2366-Tier1-PhCMV_F-type

Plasmid

#169567

Purpose

Tier-1 vector encoding PhCMV-driven F-type transactivator domain (PhCMV-F-type-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven F-type VP16

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH255-Tier1-OTetO7-PCMVmin1-SEAP

Plasmid

#169587

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-1

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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