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pLminP_Luc2P_RE3

Plasmid

#90337

Purpose

AHR - Aryl hydrocarbon receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

AHR

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163029

Purpose

FLiCRE TF for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv7-puro-OsAID (p5109)

Plasmid

#239359

Purpose

pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Rice Auxin Inducible Degradation domain

Use

Bacterial

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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p131CB10-tphKAB

Plasmid

#241911

Purpose

TPA catabolic operon under the control of a PCA inducible biosensor

Depositor

Neil Dixon

Article

Chacon et al Trends Biotechnol. 2025 Mar;43(3):647-67

Insert

tphK, tphA2, tphA3, tphB, tphA1

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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CPY-ymsfGFP

Plasmid

#209538

Purpose

Expresses CYP tagged with ymsfGFP in S. cerevisiae

Depositor

Patrick Lajoie

Article

Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

Insert

CPY

Tags

ymsfGFP

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 K222R

Plasmid

#80887

Purpose

mammalian expression of ALK7 K222R

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Tags

HA

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro

Plasmid

#135003

Purpose

Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3 GATA Luc

Plasmid

#85695

Purpose

Luciferase reporter containing three repeats of the GATA consensus site

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

3xGATA and minimal MT promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

3xGATA and minimal MT promoter

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pLminP_Luc2P_RE1

Plasmid

#90335

Purpose

NFkB - p50/p65 pathway gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NFKB

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Neo DEST (w531-1)

Plasmid

#19067

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas3

Plasmid

#209427

Purpose

Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa

Use

CRISPR and Synthetic Biology

Promoter

PtipA; crRNA under control of PermE*

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GoldenGate

Plasmid

#47388

Purpose

Expression plasmid for TALE proteins compatible with GoldenGate cloning system

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

Type

Empty backbone

Use

TALEN

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

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pNestin-EGFP

Plasmid

#38777

Depositor

Wei Cui

Article

Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

Insert

nestin 2nd intron enhancer with TK mini promoter (NES Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE27

Plasmid

#90369

Purpose

Type II Interferon - STAT1/STAT1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT1

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHIV-iRFP720-E2A-Luc

Plasmid

#104587

Purpose

Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

Depositor

Antonius Plagge

Article

Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

Insert

iRFP720-E2A-Luciferase

Use

Lentiviral and Luciferase

Tags

bi-cistronic

Expression

Mammalian

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG

Plasmid

#175503

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-mTurquoise2

Plasmid

#108235

Purpose

Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter - mTurquoise2

Expression

Bacterial

Mutation

codon optimized for experession in P. fluorescens

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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