We narrowed to 62,923 results for: uros

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132398

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

Plasmid

#176851

Purpose

lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1-5SA (YAP1 Human)

Use

Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

Plasmid

#209079

Purpose

Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

sfGFP
NGN2 (Neurog2 Mouse)

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

Plasmid

#168106

Purpose

PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

mCherry-KASH

Use

CRISPR and Cre/Lox

Promoter

CAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

Plasmid

#141370

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS

Plasmid

#191367

Purpose

Overexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dMisCas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216246

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgAAVS1.5

Use

CRISPR; Human safe harbor locus (a avs1) site-spe…

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

Plasmid

#132404

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

Plasmid

#207832

Purpose

telomere specific nuclease to create double-strand breaks at telomeres

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPB-p53RE(CDKN1A)-CMVmin-mScarlet-I-NLSx3-AU1-PGKpuro

Plasmid

#241845

Purpose

Expresses p53 transcription fluorescence reporter in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

CDKN1A promoter (CDKN1A Human)

Tags

mScarlet-I-3xNLS

Expression

Mammalian

Mutation

CDKN1A promoter (1015 bp) + CMV minimal promoter …

Promoter

n/a

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

Plasmid

#123363

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

EGFP-NLS

Use

CRISPR, Lentiviral, and Luciferase

Expression

Mammalian

Promoter

EFS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-(mcs) human CSF1 full length-IRES-Puro

Plasmid

#86797

Purpose

retroviral expression of CSF-1

Depositor

Martine Roussel, Charles Sherr

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-Puro

Expression

Mammalian

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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