We narrowed to 17,079 results for: Por

1. pRR-ERbeta-5Z

   Plasmid

   #23062

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   ERbeta (ESR2 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pCFD5-U6-3-t-attP40

   Plasmid

   #133561

   Purpose

   gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   attP40 region guide RNAs
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GNPTAB

   Plasmid

   #78108

   Purpose

   Mammalian expression of human GNPTAB with C terminal myc tag

   Depositor

   Thomas Braulke

   Article

   Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

   Insert

   GNPTAB (GNPTAB Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. TetO-FUW-VdC9BV

   Plasmid

   #62195

   Purpose

   Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

   Depositor

   Kam Leong

   Article

   Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

   Insert

   VP64dCas9BFPVP64
   

   Use

   Lentiviral

   Tags

   Two VP64s tagged to dCas9 fused to BFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive) Cas9 (dCas9)

   Promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-VHH-tev-mCherry

   Plasmid

   #117752

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-51b(+)-Caprola_13

   Plasmid

   #194674

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_13
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

   Plasmid

   #176587

   Purpose

   Bacterial expression of His14-MBP-TEV-AmaTnpB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Use

   Tags

   His14 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-51b(+)-cpHaloTag141/145

   Plasmid

   #194660

   Purpose

   Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   cpHaloTag141/145
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-51b(+)-cpHaloTag154/156

   Plasmid

   #194661

   Purpose

   Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   cpHaloTag154/156
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GRAB_eCBmut

    Plasmid

    #164605

    Purpose

    Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid non-responsive sensor GRAB_eCBmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T, F177A

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-51b(+)-Caprola_05

    Plasmid

    #194666

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcpAzpaRS_v2

    Plasmid

    #196483

    Purpose

    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40(synthetase), Pol III tRNA promoter (tRNA), C…

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Ricardo Dolmetsch, Oleksandr Shcheglovitov

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 AK3 Hyg

    Plasmid

    #37539

    Depositor

    David Sidransky

    Article

    Chang et al PLoS One. 2011;6(6):e20806. Epub 2011 Ju

    Insert

    AK3 (AK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pFUW-TetO-tdTomato-cNLS

    Plasmid

    #138532

    Purpose

    Lentiviral inducible expression of tdTomato with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-51b(+)-Caprola_01

    Plasmid

    #194662

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_CaMKIIa_SERT_EYFP

    Plasmid

    #198737

    Purpose

    Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

    Depositor

    Lin Tian

    Article

    Vargas et al Science. 2023 Feb 17;379(6633):700-706.

    Insert

    Serotonin transporter and yellow fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EFS-fDIO-GRAB_eCB2.0

    Plasmid

    #164610

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    EFS

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-DIO-GRAB_eCB2.0mut

    Plasmid

    #164607

    Purpose

    Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid non-responsive sensor GRAB_eCBmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T, F177A

    Promoter

    hSyn

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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