We narrowed to 175,616 results for: ins
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Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sigā¦ExpressionBacterial, Insect, Mammaliaā¦MutationL122A.Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXD4 (murine) MSCV
Plasmid#8549DepositorInsertHOXD4 (Hoxd4 Mouse)
UseRetroviralTagsHis and T7ExpressionMammalianMutationcontains T7 and HIS tags from pET; makes protein ā¦Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB11
Plasmid#174623PurposeOptogenetic coupling to recycling endosomes (RAB11) via iLIDDepositorInsertiLID-mCherry-RAB11 (RAB11A Synthetic, Human)
TagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB11: Met1DelPromoterChicken beta-actinAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-1(N)-SH2
Plasmid#46495DepositorInsertSHP-1(N) (PTPN6 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-109PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fes-SH2
Plasmid#46426DepositorInsertFes (FES Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 396-497PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H mā¦PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2āmPKCε-CAT-HA
Plasmid#190483PurposeExpresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAXDepositorInsertmCherry-CRY2āmPKCε-CAT-HA (Prkce Synthetic, Mouse)
TagsFused to CRY2, HA, and mCherryExpressionMammalianPromoterCMVAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promoteā¦Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
ExpressionYeastMutationF138I, T78HPromoterPMAAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-B103 (LM2907)
Plasmid#208968PurposeUnfused B103 DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-KD
Plasmid#140312PurposeExpresses HA-tagged human Src-KD in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
Tags2X HAExpressionMammalianMutationContains only the KD regionPromoterCMVAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18b
Plasmid#135554PurposeExpress rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.DepositorInsertMunc18b (Stxbp2 Rat)
Tags6xHis tag and TEV siteExpressionInsectPromoterpolyhedrin promoterAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xML1N(7Q)
Plasmid#67666PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1Nmut*2
ExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Zap70(C)-SH2
Plasmid#46533DepositorInsertZap70(C) (ZAP70 Human)
TagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 154-267PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_32B
Plasmid#75011PurposePortion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_32B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hAREG
Plasmid#199229PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertAmphiregulin preproprotein (AREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP4
Plasmid#129502PurposeExpresses AausFP4 constitutively in E. coli (most strains)DepositorInsertAausFP4
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promoteā¦Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_27B
Plasmid#75010PurposePortion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_27B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only