165,185 results

1. NM1-5S-tRNA-SgH

   Plasmid

   #128178

   Purpose

   Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   gRNA cloning cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   5S rRNA-tRNA(Arg)

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

   Plasmid

   #60661

   Purpose

   Flp-dependent expression of Kir2.1-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   FLEX- Kir2.1-T2A-tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-Cyto

   Plasmid

   #124314

   Purpose

   Expresses cytosolic specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Use

   Tags

   Cytoplasm specific seq

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRIB2 gRNA (BRDN0001144754)

   Plasmid

   #75602

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB2 (TRIB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIB2 gRNA (BRDN0001148141)

   Plasmid

   #75603

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB2 (TRIB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRECMYC-Ebu1

   Plasmid

   #240713

   Purpose

   pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

   Depositor

   Seth Shipman

   Insert

   Ebu1 RT, Eco1 ncRNA, MspRecT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Cyto-ExRai-CKAR2

   Plasmid

   #236097

   Purpose

   Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   ExRai-CKAR2-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD-puro-Cc-SARS-CoV-2_NSP16-VA

   Plasmid

   #191215

   Purpose

   Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

   Depositor

   Mohan Babu

   Insert

   Non structural protein 16 (ORF1ab SARS CoV-2)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTR01F/hFGFR2b-V5

   Plasmid

   #236014

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR2b protein

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   human FGFR2b (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   truncated CMV promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAK184.tetR.PT5-3.dCas9

    Plasmid

    #232482

    Purpose

    optimized ahTet inducible dCas9 for insertion into C. difficile genome

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Insert

    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-PT5-3

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJC.15A.sgRNA.TA

    Plasmid

    #232483

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Inserts

    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3 constitutive promoter and native promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #234754

    Purpose

    Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE_FKBP-mEGFP-WIPI2d_P2A-FRB-Fis1 (no mt-mKeima)

    Plasmid

    #223794

    Purpose

    Stable expression of protein in mammalian cell culture

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    2xFKBP-mEGFP-WIPI1-(P2A)-FRB-Fis1 (WIPI1, FIS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646)

    Plasmid

    #230884

    Purpose

    AiE1426m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Sst-Chodl neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP14202 - pAAV-AiE0743m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4202)

    Plasmid

    #224178

    Purpose

    AiE0743m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP13808 - pAAV-AiE0140h_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3808)

    Plasmid

    #224176

    Purpose

    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons (note cortical Pvalb+ and L5_ET)

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    mTFP1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human USP7_H456A-3X FLAG

    Plasmid

    #225335

    Purpose

    Lentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    USP7 (USP7 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    H456A

    Promoter

    EF1a

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP-Cyclin D2

    Plasmid

    #215051

    Purpose

    Expresses EGFP-Cyclin D2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW-LIC-N8C_Fab2b

    Plasmid

    #203692

    Purpose

    Expresses N8C_Fab2b

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab2b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge