178,376 results
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Plasmid#178335PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV CAG iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human ubiquitination-related proteins CRISPR KO library
Pooled library#174592PurposeTargets human ubiquitation-related proteins and deubiquitinating enzymes.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-F-RAM-d2tTA-TRE-mKate2
Plasmid#140274PurposeF-RAM, the Fos-dependent reporterDepositorInsertmKate2
UseAAVPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV Retrograde)
Viral prep#162378-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-sRGECO (AAV8)
Viral prep#137127-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-sRGECO (#137127). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-sRGECO plasmid DNA. EF1a-driven, Cre-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV8)
Viral prep#51502-AAV8PurposeReady-to-use AAV8 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 M
Plasmid#158078PurposeExpresses SARS-CoV-2 membrane protein in mammalian cellsDepositorInsertSARS-CoV-2 matrix (M )
ExpressionMammalianAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
COVID-SARS2 NSP13
Plasmid#159614PurposeBacterial expression plasmid for COVID-SARS2 NSP13 helicaseDepositorInsertCovid-SARS2 Nsp13
TagsHis6-Zb-TEVExpressionBacterialMutationCodon-optimized for E. coli expressionPromoterT7 - LacOAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-SomaGCaMP7
Plasmid#158759PurposeCell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseAAVExpressionMammalianPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ND4-DdCBE-right side TALE
Plasmid#157843Purposeexpresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
ExpressionMammalianAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)
Plasmid#149276PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorMutationT2318C (DNA) in PEBV coat protein sequenceAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro
Plasmid#141377PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
ExpressionMammalianPromoterU6Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLGB30
Plasmid#126620PurposeSuicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway CloningExpressionMammalianPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-RAB-EKARev
Plasmid#118474PurposeddRFP-based single-color biosensor for monitoring ERK activity.DepositorInsertRAB-EKARev
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNIL-BSD-mApple
Plasmid#124230PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M43: pHR-SFFVp-iLID::EGFP::FTH1
Plasmid#122149PurposeSelf-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.DepositorAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by