We narrowed to 18,503 results for: REV

1. pINDUCER21-puro_TAOK1

   Plasmid

   #209777

   Purpose

   doxycycline-inducible expression of TAOK1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK1 (TAOK1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AKAR4-NES

   Plasmid

   #64727

   Purpose

   Cytosol-targeted, FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

   Insert

   AKAR4-NES
   

   Tags

   Cerulean, NES, and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5FRT/TO-SAKS1-Strep-HA

   Plasmid

   #113487

   Purpose

   Expression of human SAKS1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   SAKS1 (UBXN1 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNeonGreen-C1-Nir2-LNS2

   Plasmid

   #220086

   Purpose

   C-terminal PA binding domain of Nir2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM1 (PITPNM1 Human)
   

   Tags

   NeonGreen-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 896-1216

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-UBXD4-Strep-HA

   Plasmid

   #113480

   Purpose

   Expression of human UBXD4 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD4 (UBXN2A Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-POMP

   Plasmid

   #86765

   Purpose

   N-terminal HA-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   human POMP (POMP Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-mitoGFP

    Plasmid

    #44385

    Depositor

    Pantelis Tsoulfas

    Article

    Kitay et al Hum Mol Genet. 2013 Jan 24.

    Insert

    mitoGFP (COX8A Human)
    

    Use

    Lentiviral

    Tags

    Cox8 targeting sequence and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EGFP-Cre

    Plasmid

    #86805

    Purpose

    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter

    Depositor

    Amy Lasek

    Article

    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    EGFP and nuclear localization signal: PKKKRKV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available Since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-puro_FlnaABD-TS-16flag

    Plasmid

    #234868

    Purpose

    Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid

    #234869

    Purpose

    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231346

    Purpose

    Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MCS-TurboID MSCV-IRES-GFP

    Plasmid

    #207957

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TurboID-MCS MSCV-IRES-GFP

    Plasmid

    #207958

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid

    #103865

    Purpose

    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Contains the H133A and H1058A mutations which ina…

    Promoter

    EF1a

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138150

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Mutation

    DR1 A>T

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178576

    Purpose

    AAV vector for CK(0.4)::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    AAV

    Promoter

    CamKIIa

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET21-GFP-AURKA-mCherry

    Plasmid

    #99876

    Purpose

    Expression of GFP-AURKA-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJL049

    Plasmid

    #198809

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. lenti sgRNA(MS2)_puro backbone

    Plasmid

    #73795

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-N1-STAT3

    Plasmid

    #111934

    Purpose

    Expresses human STAT3 fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Signal transducer and activator of transcription 3 (STAT3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV8R-12 Capsid

    Plasmid

    #213968

    Purpose

    AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

    Plasmid

    #198337

    Purpose

    PB plasmid expressing Halo-tagged PP7 coat protein

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    PP7 coat protein 3x Halo-GB1
    

    Use

    Piggybac

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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