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eGFP-KIF1A-Rigor

Plasmid

#172207

Purpose

Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 251 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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HypaCas9

Plasmid

#138557

Purpose

Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N692A, M694A, Q695A, H698A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

Plasmid

#161993

Purpose

Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (Snx9 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

R286A Y287A K288A

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304 PCSK5-V5 blast

Plasmid

#83100

Purpose

Lentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Flag HBx (G124L, I127A)

Plasmid

#42598

Depositor

Ding Xue

Article

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Insert

Hepatitis B virus protein X (G124L, I127A)

Tags

Flag

Expression

Mammalian

Mutation

G124L I127A

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-vDIO-mScarlet-IRES-tTA

Plasmid

#118030

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-vDIO-mScarlet-IRES-tTA

Use

AAV

Promoter

TRE

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.8

Plasmid

#102917

Purpose

expresses ABE7.8 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.8

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pEN_TT 3xFLAG ID2

Plasmid

#98390

Purpose

Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb RORgamma-t mut

Plasmid

#20127

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Mutation

RORgamma-t binding site in 0.9kb of 2kb promoter …

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
EF1a_NEUROG3_P2A_Hygro_Barcode

Plasmid

#120468

Purpose

Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Sec61b-HA-mNG21-10

Plasmid

#141213

Purpose

Membrane Tethered Split Fluorescent Reporter for ER Retragrade

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

Sec61b-HA-mNG21-10

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h1mut

Plasmid

#86690

Purpose

For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)

Tags

HA

Mutation

mutated region NHDC (asparagine 273 to cysteine 2…

Promoter

T3

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab6WT

Plasmid

#46781

Purpose

expression of WT Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 (RAB6A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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