We narrowed to 165,237 results for: ins

1. ACVR1-TAAS-R206H

   Plasmid

   #206184

   Purpose

   in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

   Depositor

   Mary Mullins

   Article

   Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

   Insert

   activin A receptor type 1 (ACVR1 Human)
   

   Use

   In vitro transcription

   Tags

   FLAG tag

   Mutation

   S190A, S192A, R206H

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTD80

   Plasmid

   #172901

   Purpose

   Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

   Depositor

   David Reiner

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   TIR-1 (TIR1 Mustard Weed)
   

   Use

   CRISPR

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RH4-bio

   Plasmid

   #47779

   Purpose

   Expresses enzymatically monobiotinylated partial RH4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RH4 (partial)
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-CRY2-P14P5K

   Plasmid

   #79570

   Purpose

   expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

   Depositor

   Olof Idevall-Hagren

   Article

   Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

   Insert

   Pip5k1c (Pip5k1c Mouse)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   R445E and R446E; truncation of amino acids 636-66…

   Promoter

   CMV

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

5. EBA181-bio

   Plasmid

   #47744

   Purpose

   Expresses enzymatically monobiotinylated full-length EBA181 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised EBA181
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS-CG-Lock-Cer-VWF

   Plasmid

   #124828

   Purpose

   Expresses multimeric human VWF-ECFP fusion protein with A2-Lock domain The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF with cyan fluorescent protein fusion between D'D3 and A1 domains and Lock A2 domain (VWF Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHub10-His-YFP-PIP2;1

   Plasmid

   #99386

   Purpose

   overexpression and isolation of recombinant At-PIP2;1 protein

   Depositor

   Ning Li

   Article

   Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

   Insert

   PIP2;1 (PIP2A Mustard Weed)
   

   Tags

   His and YFP

   Expression

   Plant

   Promoter

   2xCaMV35S

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX Slp76-SH2

   Plasmid

   #46503

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Slp76 (LCP2 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 417-533

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-mCherry-N1-iChloC_CA

    Plasmid

    #98171

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChloC_C128A gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, C128A, T159C, D156N

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-mEGFP-TEV

    Plasmid

    #105777

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mEGFP-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWPT-/mEGFP-1T-IRES-mCherry

    Plasmid

    #190190

    Purpose

    Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Mutation

    changed EGFP Kozak sequence inserting a C>T mo…

    Promoter

    EIF1-short

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-ZZ-TEV-BICDR1-GFP

    Plasmid

    #111657

    Purpose

    C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1-GFP (Bicdl1 Mouse)
    

    Tags

    8xHis-tag, GFP, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Fer-SH2

    Plasmid

    #46425

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Fer (FER Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 450-558

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEN_TT 3xFLAG-MAVS

    Plasmid

    #158628

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-mCherry-C1-Phobos

    Plasmid

    #98166

    Purpose

    blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Hck-SH2

    Plasmid

    #46445

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Hck (HCK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 134-254

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM33_Orf9b

    Plasmid

    #156485

    Purpose

    Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf9b
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-mCherry-C1-Aurora

    Plasmid

    #98167

    Purpose

    red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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