We narrowed to 12,205 results for: HAL;

1. GFP-PS9

   Plasmid

   #177944

   Purpose

   Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP (ORF1ab )
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRAR074-control-sfGFP

   Plasmid

   #185032

   Purpose

   J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Expression

   Bacterial

   Mutation

   Nucleotides of rpmE 5'UTR scrambled, Only fi…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV2-N-R203M

   Plasmid

   #177952

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203M) (N )
   

   Expression

   Mammalian

   Mutation

   R203M

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTAMAHISTEV_PfeA

   Plasmid

   #128943

   Purpose

   expresses PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75)

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL3617 (pCRISPR(BbsI)_Pse)

   Plasmid

   #200902

   Purpose

   Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER11 (miR-RUG)

   Plasmid

   #44363

   Purpose

   Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

   Depositor

   Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Insert

   PheS Gly294
   

   Use

   Lentiviral

   Available Since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINB-OTULIN (full-length, aa 1-352)

   Plasmid

   #61464

   Purpose

   Expresses human OTULIN (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

   Insert

   OTULIN (OTULIN Human)
   

   Tags

   His6-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304-VHL-IRES-GFP

   Plasmid

   #187641

   Purpose

   VHL Expression vector with GFP

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   VHL (VHL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNUT N6His hTFNG

   Plasmid

   #67240

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

   Insert

   human serum transferrin (TF Human)
   

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp

   Promoter

   SV40

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-Raichu-RhoA-CR

    Plasmid

    #40258

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Raichu-RhoA-CR (RHOA Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRY2(L348F)-CreN

    Plasmid

    #75368

    Purpose

    Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    Cre recombinase, residues 19-104

    Expression

    Mammalian

    Mutation

    L348F

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-N-HA-Pcdh15_mini_V4

    Plasmid

    #199185

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    HA-mmPcdh15_mini_V4-CD2
    

    Use

    AAV

    Tags

    Human influenza hemagglutinin (HA)

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

    Plasmid

    #52521

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA on ChR2

    Promoter

    CBA

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKan-PCUP1-AID*(N)-9myc

    Plasmid

    #99526

    Purpose

    N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SP6-VEE-GFP

    Plasmid

    #58976

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas-CmR(+)

    Plasmid

    #113633

    Purpose

    Chloramphenicol resistant derivative of pCas plasmid

    Depositor

    Olivier Lichtarge

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    Chloramphenicol resistance gene
    

    Use

    CRISPR

    Promoter

    Pcat

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCM’

    Plasmid

    #162709

    Purpose

    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Expression

    Bacterial

    Mutation

    p15A origin replaced with high-copy colE1 origin

    Promoter

    PLtet0-1 promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CibN

    Plasmid

    #209671

    Purpose

    Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

    Depositor

    Sanjeevi Sivasankar

    Article

    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Tags

    Split-TurboID-N and pmEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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