We narrowed to 10,685 results for: plasmids 101
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Plasmid#186756PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc1
Plasmid#186757PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc0
Plasmid#186758PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc2
Plasmid#186759PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc3
Plasmid#186760PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc4
Plasmid#186761PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc5
Plasmid#186762PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL003
Plasmid#180607PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertionDepositorInsertDpbCasX with R3 loop
UseCRISPRTags10x His and MBPExpressionBacterialPromoterT7Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-CD55
Plasmid#153959PurposeUsed for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequencesDepositorInsertCD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScALD6
Plasmid#126737Purposeyeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiaeDepositorInsertadh2,ald6
Tags6xHisExpressionYeastPromoterGAPAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-sLovA
Plasmid#126741Purposeyeast plasmid overexpressing sLovADepositorInsertslovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii
Tags6xHisExpressionYeastAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
St1Cas9_LMD-9_sgRNA_pUC19
Plasmid#110627PurposeU6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).DepositorInsertEmpty St1Cas9 LMD-9 sgRNA backbone
UseCRISPR and Synthetic BiologyTagsNoneExpressionMammalianPromoterhU6Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-U6/sgClta-sfGFP-Clta
Plasmid#200261PurposeU6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.DepositorInsertsfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
UseAAVAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_K95M
Plasmid#108106PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221A
Plasmid#108107PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T142Q
Plasmid#108109PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
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