169,383 results

1. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKC52

   Plasmid

   #226780

   Purpose

   Expresses Drosophila UNC-104(R348G) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (R348G) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Arginine 348 to Glycine

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKC50

   Plasmid

   #226778

   Purpose

   Expresses Drosophila UNC-104(V6M) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (V6M) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Valine 6 to Methionine

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKC28

   Plasmid

   #226776

   Purpose

   Expresses Drosophila UNC-104(G618R) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (G618R) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   G618R

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIV151

   Plasmid

   #226180

   Purpose

   Expresses Cas9 in a backbone compatible with K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAP1

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MYC-hTRAK1

   Plasmid

   #225204

   Purpose

   Expresses MYC-tagged human TRAK1

   Depositor

   Thomas Schwarz

   Article

   Pekkurnaz et al Cell. 2014 Jul 3;158(1):54-68. doi: 10.1

   Insert

   trak1 (TRAK1 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRF1 Cyterm-ymStayGold

   Plasmid

   #219855

   Purpose

   expression of Cyterm-ymStayGoldd in budding yeast

   Depositor

   Bas Teusink

   Insert

   Cyterm-ymStayGold
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBABE 3xHA-MKK6-EE puro

   Plasmid

   #216791

   Purpose

   Mammalian expression of constitutively active human MKK6

   Depositor

   Kevin Janes

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Retroviral

   Tags

   3x-HA

   Expression

   Mammalian

   Mutation

   S207E and T211E, constitutively active

   Promoter

   LTR

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC-C Sars-CoV-2 NSP12_P314L

   Plasmid

   #194813

   Purpose

   Sars-CoV-2 NSP12_P314L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP12_P314L (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P314L

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. UTS2R-DuET

    Plasmid

    #213386

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    UTS2R (UTS2R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBiT3.1-N_TLE1:1-770

    Plasmid

    #211248

    Purpose

    Mammalian expression of full-length human TLE1. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    TLE1:M1-Y770 (TLE1 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC67843

    Plasmid

    #209513

    Purpose

    Cloning in complete guide expression cassettes, with Cas12a (D156R)

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Rab13 in pCSDEST2

    Plasmid

    #140879

    Purpose

    Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab13 (rab13 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NDV-HN-SpyTag

    Plasmid

    #160000

    Purpose

    Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    NDV-HN-SpyTag
    

    Use

    Tags

    His6-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta-actin promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-puro-NKX2-8

    Plasmid

    #75080

    Purpose

    retroviral vector holding human NKX2-8 cDNA

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-8 (NKX2-8 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without native 3' UTR

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR D-TOPO-C3_38

    Plasmid

    #60279

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ACSL1 enhancer (ACSL1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL-Eco

    Plasmid

    #12371

    Depositor

    Inder Verma

    Article

    Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

    Insert

    gag/pol/env
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCL-Eco

    Plasmid

    #12371

    Depositor

    Inder Verma

    Article

    Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

    Insert

    gag/pol/env
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_BiVIPe4_Gq_dTomato

    Plasmid

    #213858

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 3X ERE TATA luc

    Plasmid

    #11354

    Purpose

    Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ERE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3X ERE TATA luc

    Plasmid

    #11354

    Purpose

    Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ERE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO.1 neo

    Plasmid

    #13425

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.

    Depositor

    Sheila Stewart

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO.1 neo

    Plasmid

    #13425

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.

    Depositor

    Sheila Stewart

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

    Plasmid

    #234826

    Purpose

    STITCHR R2Tocc editor

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    nCas9h840a-xten-R2Tocc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MoClo Tool Kit

    Plasmid Kit

    #1000000044

    Purpose

    Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators

    Depositor

    Sylvestre Marillonnet

    Available since

    April 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid

    #193298

    Purpose

    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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