163,682 results

1. pCAT218

   Plasmid

   #134886

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1974

   Plasmid

   #143988

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBX3 (TBX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTriEx-NTOM20-mVenus-Zdk2

   Plasmid

   #81011

   Purpose

   Expression of Zdk2 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk2
   

   Use

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-mito-mKate2

   Plasmid

   #174178

   Purpose

   Express mKate2 with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mKate2 with mitochondria-targeting sequence
   

   Use

   Tags

   the mitochondria-targeting sequence of the cytoch…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. RUFY1-GFP

   Plasmid

   #197394

   Purpose

   Expresses RUFY1 in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Keren-Kaplan et al Nat Commun. 2022 Mar 21;13(1):1506. doi:

   Insert

   RUFY1 (RUFY1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Mito-mEosEM

   Plasmid

   #132706

   Purpose

   application in Correlative light-electron microscopy

   Depositor

   Pingyong Xu

   Article

   Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   mitochondria targeting sequence
   

   Use

   Tags

   mEosEM

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSNAP-CLC-SNAP

   Plasmid

   #38012

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Use

   Tags

   SNAP26m

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-UCOE-SFFV-EGFP

   Plasmid

   #188900

   Purpose

   Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVoPo-mCh

   Plasmid

   #187841

   Purpose

   Constitutive mCherry-expression vector for Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid

    #71390

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-Nesprin-1αKASH

    Plasmid

    #187015

    Purpose

    Disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

    Insert

    Nesprin-1alphaKASH
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pORANGE GFP-Grin2a KI

    Plasmid

    #131486

    Purpose

    Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-HA-USP1

    Plasmid

    #22596

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP1 (USP1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21b Hot-PETase

    Plasmid

    #225761

    Purpose

    Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    Hot-PETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-HA-deLACCO1-NGR

    Plasmid

    #208024

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-OPA1

    Plasmid

    #26047

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag-FOXK2

    Plasmid

    #153133

    Purpose

    Expression of Flag tagged human FOXK2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXK2 (FOXK2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZW1-snoVector

    Plasmid

    #73174

    Purpose

    Nuclear expression of RNAs

    Depositor

    Ling-Ling Chen

    Article

    Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

    Inserts

    SNORD116-13 (SNORD116-13 Human)
    SNORD116-14 (SNORD116-14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCLASP_mScarlet_CLASP_RGS2membrane

    Plasmid

    #133086

    Purpose

    Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    mScarlet-CLASP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-ZNF598-TEV-3xFLAG

    Plasmid

    #105690

    Purpose

    Expresses C terminally 3xFLAG tagged ZNF598 protein

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    TEV-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-ABEmax-NRTH

    Plasmid

    #136922

    Purpose

    Expresses ABEmax-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMX-Baf155

    Plasmid

    #25856

    Depositor

    Hans Schöler

    Article

    Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

    Insert

    Brg1 associated factor 155 (Smarcc1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pCPRDEnv

    Plasmid

    #1732

    Purpose

    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

    Depositor

    Garry Nolan

    Insert

    Gag-Pol and Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF0208

    Plasmid

    #142556

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBTK1009 (pSL9)

    Plasmid

    #191004

    Purpose

    Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMEH343 CD63 MS2 HA

    Plasmid

    #168217

    Purpose

    Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    HA and MS2 dimer

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-CLC

    Plasmid

    #193559

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Rluc8-NbV5

    Plasmid

    #201479

    Purpose

    BRET detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-Rluc8
    

    Use

    Tags

    Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LCV2 STING KO

    Plasmid

    #217445

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting STING (STING1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge