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Showing: 1051 - 1080 of 60538 results

  1. lenti-sgRNA blast

    Plasmid
    #104993
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104993-plasmid-map-sequence-id-342099

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  2. pShuttle

    Plasmid
    #16402
    Purpose
    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only
    16402-plasmid-map-sequence-id-237978

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  3. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (AAV8)

    Viral Prep
    #65417-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (#65417). In addition to the viral particles, you will also receive purified pAAV-hSyn-dF-HA-KORD-IRES-mCitrine plasmid DNA. hSyn-driven, Cre-dependent KORD expression with bicistronic mCitrine, for SALB-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)
    Promoter
    Syn
    Tags
    mCitrine
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. pUC-L1L2

    Plasmid
    #114017
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway Cloning
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114017-plasmid-map-sequence-id-222768

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  12. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #162379-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-GFAP104-mCherry (AAV5)

    Viral Prep
    #58909-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP104-mCherry (#58909). In addition to the viral particles, you will also receive purified pAAV-GFAP104-mCherry plasmid DNA. GFAP104-driven mCherry expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc
    Promoter
    GFAP
    Tags
    mCherry
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. pCALNL-DsRed

    Plasmid
    #13769
    Purpose
    Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    DsRed2
    Use
    Cre/Lox; Flp/frt
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13769-plasmid-map-sequence-id-489004

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  18. CMV-G-GECO1.2

    Plasmid
    #32446
    Purpose
    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    G-GECO1.2
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32446-plasmid-map-sequence-id-122736

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  20. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV1)

    Viral Prep
    #162377-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  21. pAAV-CAG-GFP (AAV9 trial size)

    Viral Prep
    #37825-AAV9.T
    Purpose
    Ready-to-use AAV9 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available Since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV1)

    Viral Prep
    #26969-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV5)

    Viral Prep
    #162379-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    Dec. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1051 - 1080 of 60538 results