We narrowed to 15,156 results for: pET

1. PCx35IL-pET42

   Plasmid

   #42916

   Depositor

   John O'Brien

   Article

   Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

   Insert

   Connexin 35 cytoplasmic loop
   

   Tags

   6x His, GST, and S-tag

   Expression

   Bacterial

   Mutation

   cytoplasmic loop is aa 101-179

   Promoter

   T7

   Available Since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21b-RL018A

   Plasmid

   #42137

   Depositor

   Sheref Mansy

   Article

   Lentini et al ACS Synth Biol. 2013 Mar 15.

   Insert

   mCerulean-mCherry
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

   Plasmid

   #116966

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETcon(I-LtrI)

   Plasmid

   #41523

   Depositor

   Andrew Scharenberg

   Insert

   I-LtrI
   

   Tags

   3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-Sad1

   Plasmid

   #90297

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

   Insert

   SAD1
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-G6-mCherry

   Plasmid

   #66533

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter G6

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - G6

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-At 14-3-3 omega

   Plasmid

   #44930

   Depositor

   Robert Ferl

   Article

   Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

   Insert

   14-3-3 omega (GRF2 Mustard Weed)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET41a-MED18

   Plasmid

   #15433

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED18 (MED18 Human)
   

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET3a-SF-LMNC-TS

   Plasmid

   #169374

   Purpose

   E. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

   Depositor

   Bernd Bukau

   Article

   Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

   Insert

   LMNA residues 31-542 (LMNA Human)
   

   Tags

   Split-FlAsH Tag and TwinStrep Tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-30 (unstructured head domain…

   Promoter

   Native pET3a promotor

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30a-SRAP

    Plasmid

    #84524

    Purpose

    Expresses murine SRAP domain (250 aa) in bacteria

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    SRAP domain (Hmces Mouse)
    

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b-pknG

    Plasmid

    #102814

    Purpose

    Expression of PknG in E.coli

    Depositor

    Jean Pieters

    Article

    Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

    Insert

    pknG
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a - CPX

    Plasmid

    #89620

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Cytosolic Tryparedoxin peroxidase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-eIF4E

    Plasmid

    #37228

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    eIF4E
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pET41a iGluf

    Plasmid

    #119833

    Purpose

    Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluf
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25D

    Promoter

    T7

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET_RGG-RGG_withTEVsite

    Plasmid

    #124940

    Purpose

    Expression of RGG-RGG

    Depositor

    Benjamin Schuster, Daniel Hammer, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    with TEV site between RGG domains

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b-At 14-3-3 epsilon

    Plasmid

    #44936

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 epsilon (GRF10 Mustard Weed)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET28b-API5

    Plasmid

    #157655

    Purpose

    Expresses API5 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a(+)-HpDsbG

    Plasmid

    #39329

    Depositor

    SeWon Suh

    Article

    Yoon et al FEBS Lett. 2011 Dec 15;585(24):3862-7. E

    Insert

    oxidoreductase
    

    Tags

    His-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

19. CyPet-FKBP-E2

    Plasmid

    #175244

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet11a-ABLE

    Plasmid

    #158627

    Purpose

    Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

    Depositor

    William DeGrado

    Article

    Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

    Insert

    Apixaban-Binding Helical Bundle
    

    Tags

    6x His-tag and TEV cleavage tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET28a-BCA-P(11)4

    Plasmid

    #185780

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-P(11)4
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET15b-ITPA

    Plasmid

    #60824

    Purpose

    to purify human ITPA from bacteria

    Depositor

    Youri Pavlov

    Article

    Porta et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    ITPA (ITPA Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET31b-T7-Spinach2-Pl-B

    Plasmid

    #79161

    Purpose

    Expresses the Pl-B biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Pl-B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTS2353_Tier3(PB)-YPet_PuroR

    Plasmid

    #169652

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. CYPet-H2B-6

    Plasmid

    #55461

    Purpose

    Localization: Nucleus/Histones, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Tags

    CYPet

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. pET31b-T7-Spinach2-Pl-A

    Plasmid

    #79160

    Purpose

    Expresses the Pl-A biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Pl-A
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET26-CNTnw

    Plasmid

    #100163

    Purpose

    recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

    Depositor

    Seok-Yong Lee

    Article

    Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

    Insert

    Concentrative nucleoside transporter
    

    Tags

    MBP-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-CV-VAMP2

    Plasmid

    #168023

    Purpose

    Expresses eCFP-VAMP2(T27-R86)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP2(T27-R86)-mVenus
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET21a - SodB

    Plasmid

    #89613

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase B
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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