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Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-AsCas12j-2
Plasmid#191655PurposeAll-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2DepositorInsertAsCas12j-2
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Paxillin DN
Plasmid#186151PurposeFor expressing recombinant paxillin variants in bacteria.DepositorInsertPXN (PXN Human)
UseTagsGSTExpressionBacterialMutationPaxilln residues 1-321 added to N-terminus of WT …PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK85
Plasmid#176611PurposeTo minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.DepositorInsertFCERG
UseLentiviralTagsEGFP and HA, SNAP-tagExpressionMammalianMutationC25APromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH60
Plasmid#172142PurposeMicroscopy inclusion body assayDepositorInsertpCDF.CD
UseTagsExpressionBacterialMutationPylCD partial operon (PylB deleted to assess impa…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Adeno-miR-23b
Plasmid#112538PurposeAdenovirus overexpressionDepositorInsertmiR-23b (MIR23B Human)
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b_STS
Plasmid#139799Purposeexpresses biosynthetic enzyme STSDepositorInsertSTS (STS Mustard Weed, vitis vinifera)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P7SHb + STS:P8SHb
Plasmid#139793Purposeexpression of biosynthetic enzymes 4CL and STS in fusion with CCDepositorInserts4CL:P7SHb
STS:P8SHb
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P7S + STS:P8S
Plasmid#139794Purposeexpression of biosynthetic enzymes 4CL and STS in fusion with CCDepositorInserts4CL:P7S
STS:P8S
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P3S + STS:P4S
Plasmid#139789Purposeexpression of biosynthetic enzymes 4CL and STS in fusion with CCDepositorInsertbiosynthetic enzymes 4CL and STS in fusion with coiled-coil segments
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK504
Plasmid#71584PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges cysteine-357 to tyrosinePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SV40LT
Plasmid#136616PurposeDox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cellsDepositorInsertSV40 large T antigen
UseLentiviralTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-LRP6c
Plasmid#42960PurposeExpresses optogenetically controlled LRP6 (C-terminal domain)DepositorInsertCry2PHR-mCherry-LRP6c
UseTagsExpressionMammalianMutationLPR6 cytoplasmic domain onlyPromoterCMVAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody
Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBMNb-cALFA
Plasmid#159991PurposeFor the protein expression with ALFA tag at C-terminus in mammalian cellsDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationCMV promoter, WPREPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBJZ_003
Plasmid#155358PurposeRifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-NES-KIRIN1
Plasmid#107416PurposeMammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequenceDepositorInsertKIRIN1
UseTagsNuclear exclusion sequence (NES) (LALKLAGLDIGS)ExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only