We narrowed to 18,992 results for: Met
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Plasmid#179372PurposePC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.DepositorInsertPC-MjMAT
Tags6xHisExpressionBacterialMutationL147A, I351APromoterT7Available SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-TAG35-sfGFP
Plasmid#212120PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-TAG35-sfGFP
M. alvus Pyl-tRNA
TagsHis6 and SUMO-TAG35ExpressionBacterialMutationE35TAG (within SUMO encoding sequence)PromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK Mma PylT (CUA)
Plasmid#154782Purposeplasmid with single copy of Mma PylT (CUA anticodon)DepositorInsertMma PylT
ExpressionMammalianMutationU25CPromoterh7SKAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SBC015910
Plasmid#226287PurposeExpresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.DepositorInsertsConiferyl alcohol acyltransferase
Isoeugenol synthase 1
ExpressionBacterialAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-ChPylTMSK
Plasmid#163915PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS
Plasmid#140008PurposePlasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsert4xPylT (Mma) cassette
ExpressionMammalianMutationU25CPromoterh7SKAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mma PylRS
Plasmid#154762Purposeplasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-domain
Plasmid#200300PurposeBacterial expression plasmids for production of recombinant beta-domain of metallothionein 2aDepositorInsertbeta-domain of metallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialPromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_alpha-domain
Plasmid#200302PurposeBacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2aDepositorInsertalpha-domain of metallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialPromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K30A
Plasmid#105694PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K30A mutantDepositorInsertMetallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 30 to AlanineAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K31A
Plasmid#105695PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K31A mutantDepositorInsertMetallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 31 to AlanineAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K30A K31A
Plasmid#105696PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutantDepositorInsertMetallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 30 and 31 to AlanineAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_C21A
Plasmid#105697PurposeBacterial expression plasmids for production of recombinant metallothionein 2a C21A mutantDepositorInsertMetallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialMutationCysteine 21 to AlanineAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K20A
Plasmid#105698PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K20A mutantDepositorInsertMetallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 20 to AlanineAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_S6A
Plasmid#105699PurposeBacterial expression plasmids for production of recombinant metallothionein 2a S6A mutantDepositorInsertMetallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialMutationSerine 6 to AlanineAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS
Plasmid#140009PurposePlasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE
Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS AF
Plasmid#140023PurposePlasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationY271A, Y349FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only