Addgene

We narrowed to 17,043 results for: URE

Showing: 701 - 720 of 17043 results

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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA-Lib

Plasmid

#53121

Purpose

sgRNA expression construction in a 3rd generation lentiviral backbone

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn3beta AS4(-)

Plasmid

#58269

Purpose

Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn3beta AS4(-) (Nrxn3 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ332: pU6-GtFz1_omegaRNA-entry

Plasmid

#205265

Purpose

Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ123: pRS426-His-MBP-SpuFz1_PSP1

Plasmid

#205256

Purpose

S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pJFRC7-syt1-GCaMP6f

Plasmid

#190896

Purpose

GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

Depositor

Tom Clandinin

Article

Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

Insert

syt1-GCaMP6f

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ240: pRS426-His-MBP-MmeFz2_Native

Plasmid

#205255

Purpose

S. cerevisiae expression vector for MmeFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

Plasmid

#205261

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pKS101

Plasmid

#24631

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

FAA2 (FAA2 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pRset-OppA

Plasmid

#207869

Purpose

Bacterial expression of poly-his-tagged L. lactic OppA

Depositor

Mathew Tantama

Article

Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

Insert

OppA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP

Plasmid

#158130

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ235: pRS426-His-MBP-GtFz1_Native

Plasmid

#205254

Purpose

S. cerevisiae expression vector for GtFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1

Plasmid

#124651

Purpose

Soma-localized ChrimsonR for dense expression in excitatory neurons

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Has Service

AAV9

Insert

ChrimsonR

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CamKIIa

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_1

Plasmid

#102963

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_1 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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