We narrowed to 8,318 results for: gnal
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pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0238-ss
Plasmid#137046PurposeE. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66635.2 (BB_0238 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0238: lacks the BB0238 signal peptidePromoterT7lacAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P66-ss
Plasmid#137045PurposeE. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66949.1 (p66 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P66: lacks the P66 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P13-ss
Plasmid#137065PurposeE. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P13: lacks the P13 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
UseTagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-RevA-ss
Plasmid#137064PurposeE. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationlacks the RevA signal peptide and 5 residues (CKA…PromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-DipA-ss
Plasmid#137068PurposeE. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature DipA: lacks the DipA signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-HtrA-ss
Plasmid#137066PurposeE. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66500.2 (BB_0104 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature HtrA: lacks the HtrA signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A
Plasmid#233065PurposeExpresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.DepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsALFA and HiBiTExpressionMammalianMutationLeucine 928 to Alanine; Methionine 929 to AlaninePromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NRF2-E79Q
Plasmid#207813PurposeN-terminal tagged NRF2-E79Q for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-NRF2-E79Q (NFE2L2 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationE79QPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-NRF2-E79Q-V5-miniTurbo
Plasmid#207814PurposeC-terminal tagged NRF2-E79Q for miniTurbo proximity experimentsDepositorInsertNRF2-E79Q-V5-miniTurbo (NFE2L2 Human)
UseLentiviralTagsV5-miniTurboExpressionMutationE79QPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-ΔPS
Plasmid#214901Purposeexpression of the truncated variants of the SHIP2 without phosphatase domainDepositorInserthuman SHIP2 delta aa 419-739 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 419-739aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-ΔPR1
Plasmid#214902Purposeexpression of the truncated variants of the SHIP2 without first proline-rich domainDepositorInserthuman SHIP2 delta aa 123-410 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 123-410aaPromoterCMVAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-C2
Plasmid#214906Purposeexpression of the truncated variants of the SHIP2 that retains second proline-rich domainDepositorInserthuman SHIP2 aa 740-1189 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 1-739, 1190-1258aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-C1
Plasmid#214905Purposeexpression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motifDepositorInserthuman SHIP2 aa 740-1258 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 1-739aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-N2
Plasmid#214904Purposeexpression of the truncated variants of the SHIP2 that retains first proline-rich domainDepositorInserthuman SHIP2 aa 123-418 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 1-122, 419-1258aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-N1
Plasmid#214903Purposeexpression of the truncated variants of the SHIP2 that retains only SH2 domain and first proline-rich domainDepositorInserthuman SHIP2 aa 1-418 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 419-1258aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-bio-myc-cCE
Plasmid#112711PurposeFor tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsHIV Rev nuclear localization signal (NES), Stop c…ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMV with Tet repressor binding sitesAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
G15-CASE
Plasmid#168126PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at E244/N245 within GNA15PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only
