We narrowed to 24,002 results for: nts

1. pNTI934

   Plasmid

   #216637

   Purpose

   Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   Cal-ID-PM
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNTI821

   Plasmid

   #216630

   Purpose

   Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   Cal-ID
   

   Use

   Sleeping beauty transposon system

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNTI822

   Plasmid

   #216631

   Purpose

   Tet-inducible cpTurboID (V5) for Sleeping Beauty transposon system

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   cpTurboID
   

   Use

   Sleeping beauty transposon system

   Tags

   V5

   Expression

   Mammalian

   Mutation

   L120P

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC57-HBB-nLuc

   Plasmid

   #175432

   Purpose

   Used to generate IVT RNA for translation extracts

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   HBB-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC Ad VAI

   Plasmid

   #175518

   Purpose

   For mammalian expression of adenovirus VAI RNA

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   Ad VAI RNA
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   lac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNTI827

   Plasmid

   #216635

   Purpose

   Cal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   Cal-ID-ERM
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BPK2139

   Plasmid

   #65776

   Purpose

   Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNTI823

    Plasmid

    #216632

    Purpose

    Cal-ID variant with T195/G196 split site, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID (split site variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNTI828

    Plasmid

    #216636

    Purpose

    cpTurboID-ERM (V5) ER membrane-localized cpTurboID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID-ERM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNTI935

    Plasmid

    #216638

    Purpose

    cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID-PM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO APEX2-eIF4E1

    Plasmid

    #129644

    Purpose

    Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    EIF4E1 (EIF4E Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer-rat EF1 alpha

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1-ZNF746

    Plasmid

    #207825

    Purpose

    Dox-inducible expression of ZNF746

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746 (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNTI826

    Plasmid

    #216634

    Purpose

    cpTurboID (V5) for AAV production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JEH127

    Plasmid

    #179299

    Purpose

    Expresses dCas9 fused to VPR

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge