We narrowed to 7,733 results for: CCH

Showing: 7201 - 7220 of 7733 results

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pYO379

Plasmid

#235733

Purpose

Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Tags

3xTEV-ZZ and ZZ-3xTEV

Expression

Yeast

Mutation

S2A on ScVPS34

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar2

Plasmid

#232739

Purpose

Expresses NADPH/NADP+ biosensor NAPstar2 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar2

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar4

Plasmid

#232741

Purpose

Expresses NADPH/NADP+ biosensor NAPstar4 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar4

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar6

Plasmid

#232742

Purpose

Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar6

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar7

Plasmid

#232744

Purpose

Expresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar7

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221112

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

STE3

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-α-Synuclein-c_myc

Plasmid

#225222

Purpose

Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

α-Synuclein
Aga2

Tags

GS linker, HA Tag, and c-myc Tag

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTT021

Plasmid

#226782

Purpose

Expression of wzxE and other genes from the E. coli enterobacterial common antigen biosynthesis gene cluster

Depositor

James Naismith, Chris Whitfield

Article

Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

Insert

wzxE, wecF, wzyE, wecG

Expression

Bacterial

Promoter

BAD

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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macroH2A1.1-GFP (pc2188)

Plasmid

#223707

Purpose

Expression of macroH2A1.1 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.1-GFP (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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macroH2A1.2-GFP (pc2189)

Plasmid

#223708

Purpose

Expression of macroH2A1.2 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.2-GFP (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-GAL4-PARP1-CAT-L713F

Plasmid

#175949

Purpose

Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

GAL4 DNA binding domain (GAL4 Budding Yeast)

Tags

6xHis tag

Expression

Bacterial

Mutation

L713F, V762A for the 'PARP1-CAT-L713F' …

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pIRHDGAL43C

Plasmid

#218279

Purpose

Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)

Expression

Yeast

Mutation

GAL3 (S509P), DHFR (P66L)

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pIRHDGAL3C

Plasmid

#218276

Purpose

Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)

Expression

Yeast

Mutation

GAL3 (S509P), DHFR (P66L)

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-mCherry-NAP1 (WT)

Plasmid

#198036

Purpose

Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV and mCherry

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m-ΔZF

Plasmid

#192276

Purpose

Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

Cas12m ΔZF

Use

CRISPR

Expression

Bacterial

Mutation

H459A, C552A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

Plasmid

#198072

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

TBK1ΔCTD (res 1-666)

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pROS13-Tps2/Gsy1

Plasmid

#196613

Purpose

Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

S000001911 (TPS2, GSY1 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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