We narrowed to 18,503 results for: REV

1. pHis APEX2-eIF4A1

   Plasmid

   #129645

   Purpose

   Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   eukaryotic translation initiation factor 4A1 (EIF4A1 Human)
   

   Tags

   6xHis and APEX2

   Expression

   Bacterial

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

   Plasmid

   #89149

   Purpose

   An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. YFP-CD82

   Plasmid

   #1819

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   CD82 (CD82 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Lyso-ExRai-AktAR2

   Plasmid

   #184052

   Purpose

   Lysosome-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-ExRai-AktAR2
   

   Tags

   Lysosome-associated membrane protein 1 (LAMP1).

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pADSL-EGFP

   Plasmid

   #99109

   Purpose

   Expresses human ADSL-EGFP fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   An et al Science. 2008 Apr 4;320(5872):103-6. doi

   Insert

   ADSL (ADSL Human)
   

   Tags

   EGFP (enhanced green fluorescent protein)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330 _CRISPIE donor B7

   Plasmid

   #172848

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-FKBP-PTEN

   Plasmid

   #116866

   Purpose

   Recruitable PTEN dual-specificity phosphatase

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PTEN (PTEN Human)
   

   Tags

   FKBP12 and mCherry

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-GfaABC1D-GRAB_Ado1.0med

   Plasmid

   #153289

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Promoter

   GfaABC1D

   Available Since

   Aug. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXD71Cas10.0-Pct5.1-crRNA(AarI)

   Plasmid

   #191633

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINK-OTUB1 (full-length, aa 1-271)

    Plasmid

    #61420

    Purpose

    Expresses human OTUB1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV10.F3

    Plasmid

    #63199

    Purpose

    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. beta Synuclein-VC

    Plasmid

    #89484

    Purpose

    expresses human beta Synuclein fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CBX3 A6.5 gRNA

    Plasmid

    #90569

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CBX3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CBX3 (Guide Designation A6.5)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-MAP1LC3C

    Plasmid

    #137758

    Purpose

    Expression of LC3C

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3C (MAP1LC3C Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CBX3 A5.5 gRNA

    Plasmid

    #90568

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CBX3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CBX3 (Guide Designation A5.5)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFlp-Gm

    Plasmid

    #222335

    Purpose

    Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Promoter

    cl, flp, and sacB

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138145

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330 _CRISPIE donor B9

    Plasmid

    #172850

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_SC_4.26_STARR-seq

    Plasmid

    #220220

    Purpose

    AAV-STARR-seq screening vector with 4.26 promote

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    4.26

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEGFP-HRas S17N

    Plasmid

    #18665

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    S17N mutation in H-Ras

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. ER-NFAST

    Plasmid

    #233596

    Purpose

    Expression of NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    NFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV7.1_3xFLAG-TAOK1

    Plasmid

    #172991

    Purpose

    constitutive expression of FLAG-tagged TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK1 (TAOK1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid

    #86843

    Purpose

    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

    Depositor

    Thomas Cech

    Article

    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

    Insert

    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    

    Tags

    FLAG-HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous TERT promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSS146

    Plasmid

    #64770

    Purpose

    For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Promoter

    TDH4

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. VPS26C_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176982

    Purpose

    Mammalian expression vector encoding VPS26C and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    VPS26C (VPS26C Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. VN-beta Synuclein

    Plasmid

    #89483

    Purpose

    expresses human beta Synuclein fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. RRM1 E1.3 gRNA

    Plasmid

    #90880

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E1.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA5FRT/TO-UBXD3-Strep-HA

    Plasmid

    #113488

    Purpose

    Expression of human UBXD3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD3 (UBXN10 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. mCerulean3-Mito-7

    Plasmid

    #55433

    Purpose

    Localization: Mitochondria, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Mito (COX8A Human)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pAF123

    Plasmid

    #135921

    Purpose

    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Available Since

    Jan. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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