We narrowed to 31,225 results for: multi
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Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W
Plasmid#67975PurposeCRISPR gRNA expression vector with an improved scaffold and puro/ZsG markersDepositorInsertU6gRNA cassette, PGKpuro2AZsG cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFREE_Cm
Plasmid#92052PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
gRNA array
UseCRISPRPromoterpRhamBAD and ptetAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
miReporter-PGK
Plasmid#82477PurposemicroRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.DepositorInsertH2B-mCherry and H2B-Citrine. HygroR
Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTOX12
Plasmid#127457PurposeEncodes Tse2 toxin. Gent-RDepositorInserttse2 toxin
ExpressionBacterialPromoterpRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd:TRE-gateway
Plasmid#30217DepositorTypeEmpty backboneUseAdenoviralAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)
Plasmid#105200PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(HC)-P2A-antiPD1(LC)
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLH-sgRNA1-boxB-MS2-PP7
Plasmid#75397PurposesgRNA1-boxB-MS2-PP7DepositorInsertsgRNA1-boxB-MS2-PP7
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pComb3HSS
Plasmid#64133Purpose2nd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTagsgeneIIIExpressionBacterialPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2HLacZpA1I.7 (#59)
Plasmid#35965DepositorInsertsAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)
Plasmid#85798PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingMutationhomology arms for knock-in into first intronAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
ExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDMD-puro
Plasmid#88895Purpose28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianMutationcodon optimizedAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
ExpressionBacterial and WormPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-yoPA-mCherry-Kan
Plasmid#44950PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selectionDepositorInsertyoPA-mCherry
ExpressionYeastAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD268
Plasmid#132523PurposemNG^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mNGExpressionWormAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)
Plasmid#73476PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-GFP-P2A-N2c(G)
UseAAVExpressionMammalianAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BioID-FAM21
Plasmid#121046PurposeExpresses GFP tagged BioID and human FAM21C in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsGFPExpressionMammalianMutationR118G (highly promiscuous form)PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
Tagslinked to red flouresent protein gene (katushka)ExpressionMammalianPromoterCAGAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by