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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,241 results for: multi

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Showing: 7241 - 7260 of 18241 results

  1. ADORA2A-DuET

    Plasmid
    #213174
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADORA2A (ADORA2A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213174-plasmid-map-sequence-id-430742

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  4. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Publication
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  6. pZY102

    Plasmid
    #73933
    Purpose
    This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.
    Depositor
    Zhanyuan Zhang
    Publication
    Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E
    Inserts
    bar
    GUS
    Expression
    Plant
    Mutation
    intron is inserted into the GUS coding region
    Promoter
    35S
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    73933-plasmid-map-sequence-id-506143

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  8. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197064
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Publication
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197064-plasmid-map-sequence-id-402605

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  11. GRM2-DuET

    Plasmid
    #213301
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM2 (GRM2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213301-plasmid-map-sequence-id-430907

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  19. F2R-DuET

    Plasmid
    #213234
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    F2R (F2R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213234-plasmid-map-sequence-id-430897

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Showing: 7241 - 7260 of 18241 results