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FMR1-E17B-P2A-NLuc

Plasmid

#157857

Purpose

donor plasmid for FMR1-Nluc

Depositor

Xinyu Zhao

Article

Li et al Stem Cells. 2017 Jan;35(1):158-169. doi:

Insert

FMR1-Nlu (FMR1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

Plasmid

#163550

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Mm_MyoGEF(WT)

Plasmid

#136327

Purpose

Mouse MyoGEF-WT in Gateway pENTR

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Gateway entry vector

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT-TO-Nprot_GC3opt

Plasmid

#157730

Purpose

mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot_GC3opt (N SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp8_GC3opt

Plasmid

#157697

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt

Plasmid

#157700

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp8_GC3opt

Plasmid

#157698

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ID1GFP Donor

Plasmid

#119282

Purpose

Mouse ID1-eGFP for HR to create reporter

Depositor

Joan Massague

Article

Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

Insert

Id1GFP + homology arms (Id1 Mouse)

Use

Reporter

Tags

eGFP

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 WT

Plasmid

#129292

Purpose

Gateway entry clone encoding wild-type human ATG3 with a stop codon

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 K243R

Plasmid

#129294

Purpose

Gateway entry clone encoding human ATG3 K243R

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Mutation

changed Lysine 243 to Arginine

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236H

Plasmid

#124616

Purpose

Chimeric ETS domain: N236H

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (ETS1, SPI1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a histidi…

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236Q

Plasmid

#124615

Purpose

Chimeric ETS domain: N236Q

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a glutami…

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236Y

Plasmid

#124614

Purpose

Chimeric ETS domain: N236Y

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a Tyrosine

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1-W300A

Plasmid

#123301

Purpose

Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

W300A

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3A

Plasmid

#114054

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3A (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3D

Plasmid

#114055

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3D (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pFL His-I3 V41A / W43A

Plasmid

#113515

Purpose

Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Tags

6*His

Expression

Insect

Mutation

V41A /W43A

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3D

Plasmid

#114058

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3D (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670D, S676D and T680D

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3A

Plasmid

#114059

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3A (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670A, S676A and T680A

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154A/H250Y

Plasmid

#108491

Purpose

expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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