We narrowed to 64,643 results for: cell;

1. pcDNA3.1-OGT_opt

   Plasmid

   #190858

   Purpose

   For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells.

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3-H-Ras_V12

   Plasmid

   #39504

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS V12 (HRAS Human)
   

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-NRCH-PE2max-BSD

   Plasmid

   #191103

   Purpose

   Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   NRCH-PEmax
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9-NRCH

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRV.GFP FOXP3

   Plasmid

   #13250

   Depositor

   Anjana Rao

   Article

   Wu et al Cell. 2006 Jul 28. 126(2):375-87.

   Insert

   FOXP3 (FOXP3 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-UBC-mCD24-T2A-GFP

   Plasmid

   #205448

   Purpose

   lentiviral plasmid for expression of mouse CD24

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD24 (Cd24a Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGB_YFP-MiniTurboID

   Plasmid

   #222433

   Purpose

   Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX317 GFP

   Plasmid

   #193681

   Purpose

   Constitutive lentiviral expression of GFP

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   GFP
   

   Use

   Lentiviral

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ER-HA-mNG21-10

   Plasmid

   #141165

   Purpose

   Split Fluorescent Reporter for ER Retrograde Trafficking

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   ER-HA-mNG21-10-KDEL
   

   Use

   Lentiviral

   Tags

   Signal Sequence, KDEL and HA

   Expression

   Mammalian

   Mutation

   K128M, S142T, R150M, G172V and K213M

   Promoter

   Ef1a

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-KDM4A

   Plasmid

   #188233

   Purpose

   Express human KDM4A in mammalian cells

   Depositor

   Young Tang

   Article

   Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

   Insert

   Lysine-specific demethylase 4A (KDM4A Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

    Plasmid

    #86421

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-KAR2SS-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SnapTag-Binder

    Plasmid

    #179142

    Purpose

    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Tags

    SNAP-tag

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-Pickles 2.34NES

    Plasmid

    #100028

    Purpose

    Improved FRET biosensor for the measurement of BCR-ABL activity.

    Depositor

    Yusuke Ohba

    Article

    Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

    Insert

    Pickles-2.34NES
    

    Use

    Pfx vector is derived from pcmv vector.

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62904

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3XFlag pCMV5-TOPO TAZ WT

    Plasmid

    #24809

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid

    #140022

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    PcKRS (pylS Methanosarcina bakeri)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    M241F, A267S, Y271C, L274M

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62949

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX317 PRDM14

    Plasmid

    #193682

    Purpose

    Constitutive lentiviral expression of PRDM14

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-mCherry-Binder

    Plasmid

    #179139

    Purpose

    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPS

    Plasmid

    #85801

    Purpose

    Backbone for pooled cloning of Perturb-seq libraries

    Depositor

    Aviv Regev

    Article

    Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. vDip-C: pAAV-Pcp2-mGreenLantern-KASH

    Plasmid

    #207613

    Purpose

    vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

    Depositor

    Longzhi Tan

    Article

    Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    KASH

    Promoter

    mouse Pcp2 promoter (L7-6)

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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