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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,479 results for: dykddddk

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Showing: 7301 - 7320 of 14479 results

  4. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  5. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  12. HsCERK

    Plasmid
    #118600
    Purpose
    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate
    Depositor
    Kevin Lynch
    Publication
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens ceramide kinase
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available since
    Feb. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    118600-plasmid-map-sequence-id-234128

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  13. HsCERK

    Plasmid
    #118600
    Purpose
    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate
    Depositor
    Kevin Lynch
    Publication
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens ceramide kinase
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available since
    Feb. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    118600-plasmid-map-sequence-id-234128

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  14. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135014-plasmid-map-sequence-id-267599

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  15. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135014-plasmid-map-sequence-id-267599

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  16. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid
    #135012
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    135012-plasmid-map-sequence-id-267596

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  17. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid
    #135012
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    135012-plasmid-map-sequence-id-267596

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  20. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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Showing: 7301 - 7320 of 14479 results