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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 30,814 results for: his

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Showing: 7301 - 7320 of 30814 results

  4. gWiz_BM40-vAchT-Cytosolic-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218446
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-Vh-hIgG1-CH6
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218446-plasmid-map-sequence-id-434499

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  6. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid
    #169194
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells
    Depositor
    John Diffley
    Article
    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531
    Insert
    3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    3xFlag-His6
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169194-plasmid-map-sequence-id-333980

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  7. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid
    #153446
    Purpose
    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.CohE
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153446-plasmid-map-sequence-id-306487

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  13. HOXA6 (murine, partial) HIS-tag pET

    Plasmid
    #8558
    Depositor
    Corey Largman
    Insert
    HOXA6 (Hoxa6 Mouse)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    partial cDNA encoding homeodomain and preceeding …
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  15. pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

    Plasmid
    #92225
    Purpose
    Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    deGFP Y35TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue Y35 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92225-plasmid-map-sequence-id-197052

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  17. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

    Plasmid
    #44081
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Available Since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44081-plasmid-map-sequence-id-61675

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  19. pMAL-c2X_MBP-LcgcN(C24S)-H6

    Plasmid
    #245527
    Purpose
    Maltose binding protein N-terminally fused to His-tagged N-terminal LCGC14 PolB2split intein fragment
    Depositor
    Henning Mootz
    Article
    Humberg et al Angew Chem Int Ed Engl. 2025 Oct 23:e202
    Insert
    N-terminal Fragment of the LCGC14 Split Intein with C24S Mutation
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    C24S within the N-terminal fragment of the LCGC14…
    Promoter
    tac
    Available Since
    Nov. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    245527-plasmid-map-sequence-id-492806

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Showing: 7301 - 7320 of 30814 results