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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7321 - 7340 of 9221 results

  2. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163127-plasmid-map-sequence-id-331491

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  6. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

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  7. pENTR-R4R3-stop

    Plasmid
    #113745
    Purpose
    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    stop cassette
    Use
    CRISPR and Gateway Cloning
    Mutation
    Added 3 stop codons to the original sequence; see…
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113745-plasmid-map-sequence-id-260896

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  10. pJAT7

    Plasmid
    #204292
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204292-plasmid-map-sequence-id-405844

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  12. p5155

    Plasmid
    #175391
    Purpose
    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)
    Depositor
    Michael Wiles
    Article
    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10
    Type
    Empty backbone
    Use
    Mouse Targeting; Cloning vector, to be used to de…
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    175391-plasmid-map-sequence-id-348372

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  14. pOCC167

    Plasmid
    #118909
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118909-plasmid-map-sequence-id-232266

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Showing: 7321 - 7340 of 9221 results