We narrowed to 1,917 results for: FRT

1. pCDNA5/FRT-ChemoB-NAD

   Plasmid

   #193824

   Purpose

   CMV overexpression of ChemoB-NAD in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoB-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EBFP2:N39Y, A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_Lyn11-STf

   Plasmid

   #182008

   Purpose

   CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Lyn11-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO/Flag-mKO2

   Plasmid

   #110371

   Purpose

   Mammalian expression of mKO2 with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   monomeric Kusabira Orange 2
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

   Plasmid

   #157699

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

   Plasmid

   #157692

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA5/FRT/TO GFP DNAJB7

   Plasmid

   #19503

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB7 (DNAJB7 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TetO-fSNAPc

   Plasmid

   #106986

   Purpose

   open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   fSNAP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

   Plasmid

   #124864

   Purpose

   Mutagenesis of Grin2b

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2b (Grin2b Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p{3P3-dsRed::FRT-RC,attB}

   Plasmid

   #182177

   Purpose

   fluorescent reporter gene with yeast recombination site within intron

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   DsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p{ie1-eGFP::FRT-RC,attB}

    Plasmid

    #182175

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p{3P3-mTurquoise::FRT-RC,attB}

    Plasmid

    #182181

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    mTurquoise
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGE-attBCC-FRT-mW (pTL788)

    Plasmid

    #115159

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-CC FRT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT366_pattB-Hb9-GFP-pA-FRT5

    Plasmid

    #52552

    Purpose

    for PhiC31 integrase-mediated integration of pHb9-GFP

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA-FRT-FAK-kinase0

    Plasmid

    #59122

    Purpose

    FAK fret control sensor without FERM domain with no linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCMV:ECFP(loxP)(FRT)HNF1B

    Plasmid

    #31439

    Depositor

    Gerhart Ryffel

    Article

    Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. LCMV:ECFP(loxP)(FRT)MCS

    Plasmid

    #31377

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    ECFP(loxP)MCS
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid

    #80272

    Purpose

    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF3-CAG-Tol-FRT

    Plasmid

    #141349

    Purpose

    Shuttling cassette containing the Tol ORF

    Depositor

    Ton Schumacher

    Article

    Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

    Insert

    Tol cassette
    

    Use

    Mouse Targeting

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 FRT/TO MycLAP-STIL ∆CC

    Plasmid

    #80268

    Purpose

    mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    missing conserved coiled-coil (amino acids 721-74…

    Promoter

    CMV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5 FRT TO myc p31Comet

    Plasmid

    #59833

    Purpose

    Allows the integration of myc p31Comet in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Westhorpe et al J Cell Sci. 2011 Nov 15;124(Pt 22):3905-

    Insert

    p31 (MAD2L1BP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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