We narrowed to 757 results for: PI
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Plasmid#169841PurposeExpress a pegRNA used for correction (via A•T-to-G•C) of the E342K mutationDepositorInsertpegAAT K342E correction (SERPINA1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45
Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorInsertCdc45 (cdc45.L Frog)
UseTagsFLAG and HisExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45-6His
Plasmid#211379Purposebacterial expression of human Cdc45 C-terminally fused to 6xHis-TagDepositorInsertCdc45 (CDC45 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgAAT Nicking
Plasmid#169842PurposeExpress a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutationDepositorInsertsgAAT Nicking (SERPINA1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS1[RbsR-L,LacI-L]
Plasmid#60757PurposeContains PIq driving expression RbsR-L, and PI driving expression of LacI-L.DepositorInsertsRbsR-L
Dimeric LacI
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. LacI DBD with RbsR L…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-DIO-intron336-dLUC
Plasmid#110056PurposeCry1 transcription luciferase reporterDepositorInsertCry1 promoter and luciferase (Cry1 Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCry1Available sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-forward-intron336-Venus-NLS-D2
Plasmid#110054PurposeCry1 transcription fluorescence reporterDepositorInsertCry1 promoter and Venus (Cry1 Mouse)
UseAAVTagsNLS-D2ExpressionMammalianMutationPromoterCry1Available sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-TAPP1-PH
Plasmid#67662PurposeMammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-TAPP1-PH
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_D14_3Flag
Plasmid#182495PurposeContains cDNA of the MET gene without the exon 14DepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationExon 14 deletedPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-PH-TAPP1
Plasmid#161985PurposeExpresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)DepositorInsertPH-TAPP1 (PLEKHA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-PI35P2
Plasmid#92419PurposeFluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.DepositorInsertMCOLN1 (Mcoln1 Mouse)
UseTagseGFPExpressionMammalianMutationSynthetic gene, codon optimized for human.PromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2
Plasmid#110055PurposeCry1 transcription fluorescence reporterDepositorInsertCry1 promoter and Venus (Cry1 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianMutationPromoterCry1Available sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCALPs-HA-NFAT1 (4-460)-GFP
Plasmid#88879PurposeLentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFPDepositorInsertHA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
UseLentiviralTagsGFP fusion protein and HA tagExpressionMammalianMutationamino acids 4-460 only; L78P & L287P *see bel…PromoterSFFV (spleen focus forming virus)Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
2PH-PLCd-APEX2-P2A-mKate2
Plasmid#71284PurposeMammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporterDepositorInsert2PH-PLCd-APEX2-P2A-mKate2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZS1[TreR-T]
Plasmid#60754PurposeContains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.DepositorInsertsTreR-T
GalS-T
UseSynthetic BiologyTagsExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xML1N(7Q)
Plasmid#67666PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1Nmut*2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-T,LacI-T]
Plasmid#60768PurposeContains PIq driving expression FruR-T, and PI driving expression of LacI-T.DepositorInsertsFruR-T
Dimeric LacI with the TAN DBD
UseSynthetic BiologyTagsExpressionBacterialMutationDimeric LacI with the TAN DBD. and LacI/GalR repr…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T,TreR-T]
Plasmid#60775PurposeContains PIq driving expression GalS-T, and PI driving expression of TreR-T.DepositorInsertsGalS-T
TreR-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with GalS LB…PromoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only