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AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

Plasmid

#201032

Purpose

Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wNIR-GECO2-T2A-HO1

Plasmid

#159606

Purpose

Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

wNIR-GECO2-T2A-HO1

Expression

Worm

Promoter

tag-168

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)(mature peptide)-pcw107-V5

Plasmid

#64643

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Mutation

G23V

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)(mature peptide)-pcw107

Plasmid

#64642

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
TGFbetaR1 (T204D)-pcw107-V5

Plasmid

#64629

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TGFBR1 (transcript variant 1) (TGFBR1 Human)

Use

Lentiviral

Tags

V5

Mutation

T204D

Promoter

PGK

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
JAK2 (V617F)-pcw107-V5

Plasmid

#64610

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

JAK2 (JAK2 Human)

Use

Lentiviral

Tags

V5

Mutation

V617F

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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Caspase-8 (C360A)-pcw107-V5

Plasmid

#64632

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CASP8 (transcript variant B) (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pThermoCas9i_ctrl

Plasmid

#100982

Purpose

Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

Depositor

John van der Oost

Article

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Tags

None

Expression

Bacterial

Mutation

changed aspartate 8 to alanine and histidine 582 …

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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GSK3beta (K85A)-pcw107-V5

Plasmid

#64614

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

GSK3B (GSK3B Human)

Use

Lentiviral

Tags

V5

Mutation

K85A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
BCL2-pcw107-V5

Plasmid

#64630

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

BCL2 (transcript variant alpha) (BCL2 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
AR-V7-pcw107

Plasmid

#64635

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Mutation

splice isoform*

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

Plasmid

#64613

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Tags

V5

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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MEK1 (S218D,S222D)-pcw107

Plasmid

#64604

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S218D, S222D

Promoter

PGK

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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Gli2 truncation-pcw107-V5

Plasmid

#64626

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

GLI2 (truncation mutant) (GLI2 Human)

Use

Lentiviral

Tags

V5

Mutation

truncated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
beta-catenin (S33Y)-pcw107

Plasmid

#64615

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Mutation

S33Y

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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SmoM2 (W535L)-pcw107-V5

Plasmid

#64628

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

SMO (SMO Human)

Use

Lentiviral

Tags

V5

Mutation

W535L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
Kras (G12V)-pcw107

Plasmid

#64602

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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