We narrowed to 18,683 results for: met

1. pAS_4xMma PylT_FLAG-Mma PylRS AF

   Plasmid

   #140023

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y271A, Y349F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xMma PylT_FLAG-Mma PylRS

   Plasmid

   #140009

   Purpose

   Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MT2A_beta-Villin

   Plasmid

   #200301

   Purpose

   Bacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35

   Depositor

   Artur Krężel

   Article

   Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

   Insert

   beta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)
   

   Use

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

   Plasmid

   #61629

   Purpose

   R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   mDII
   ntdTomato
   DII
   n3xVenus
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172318

   Purpose

   CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

   Plasmid

   #61628

   Purpose

   Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   ntdTomato
   n2xEGFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   2 copies of EGFP with N-terminal SV40 NLS

   Promoter

   Auxin-dependent DR5 promoter containing 9 reverse…

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-dCas13-M3nls

   Plasmid

   #155366

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Promoter

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM1

   Plasmid

   #51401

   Purpose

   Expresses truncated version of NLL-MetRS from endogenous MetG promoter

   Depositor

   David Tirrell

   Article

   Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

   Insert

   MetRS (metG E coli)
   

   Use

   Tags

   Expression

   Mutation

   L13N, Y260L, H301L; Please note that a truncated …

   Promoter

   MetG

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xMma PylT(UUA)_FLAG-AcKRS

    Plasmid

    #140010

    Purpose

    Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. prhaBAD-pA-Hia5

    Plasmid

    #222303

    Purpose

    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    pA-Hia5
    

    Use

    Tags

    6His and protein A

    Expression

    Bacterial

    Mutation

    codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF6-Acly(S455D)

    Plasmid

    #70768

    Purpose

    Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Use

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455D

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE-Tight-Rluc8

    Plasmid

    #79844

    Purpose

    Rluc8 reporter plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    Rluc8
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mT-Sapphire-N1

    Plasmid

    #54569

    Purpose

    Localization: N1 Cloning Vector, Excitation: 399, Emission: 511

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mT

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. mT-Sapphire-C1

    Plasmid

    #54545

    Purpose

    Localization: C1 Cloning Vector, Excitation: 399, Emission: 511

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mT-Sapphire

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAH01

    Plasmid

    #128162

    Purpose

    IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

    Depositor

    Michael Guarnieri, Calvin Henard

    Article

    Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJC53.2/PRMT5

    Plasmid

    #35692

    Depositor

    Phillip Newmark

    Article

    Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

    Insert

    Protein arginine methyltransferase 5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Sp6 for sense, T7 for sense and antisense

    Available Since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKA-141

    Plasmid

    #79832

    Purpose

    Expresses RpBphP1-mCherry

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    BphP1-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd1

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mT-Sapphire-pBAD

    Plasmid

    #54571

    Purpose

    Localization: Protein Expression Vector , Excitation: 399, Emission: 511

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    6xHis and mT-Sapphire

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits