161,697 results

1. pLM-AMA15.0 Cas9

   Plasmid

   #138944

   Purpose

   Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

   Insert

   p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
   

   Use

   CRISPR and Synthetic Biology; Ama1 autonomously r…

   Tags

   NLS

   Expression

   Mutation

   Promoter

   p40S (AN0465) Aspergillus nidulans

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-PtenWT-Puro

   Plasmid

   #135676

   Purpose

   Constitutive expression (cMV promoter) of wildtype Pten cDNA, Puromycin selection

   Depositor

   Florian Karreth

   Insert

   Pten (Pten Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-CEP41_Halo

   Plasmid

   #135446

   Purpose

   Mamalian cell expression of HaloTag7 localized at the centrosome

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   CEP41-HaloTag7
   

   Use

   Tags

   CEP41

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-Flag-hsDicer

   Plasmid

   #41584

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Sac-Abeta(M1-42)

   Plasmid

   #71875

   Purpose

   Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-42) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPXL

   Plasmid

   #12257

   Purpose

   2nd generation lentiviral transfer plasmid

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Beta-Catenin-20

   Plasmid

   #55001

   Purpose

   Localization: Tight Junctions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Beta-Catenin (Ctnnb1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Rab27B

   Plasmid

   #89447

   Purpose

   Expression of GFP-tagged human Rab27B in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B (RAB27B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-Coff/Fon-GCaMP6M (AAV8)

   Viral Prep

   #137121-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6M (#137121). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6M plasmid DNA. EF1a-driven expression of GCaMP6m in the presence of Flp recombinase (inhibited in the presence of Cre recombinase). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   None

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

10. NW11

    Plasmid

    #133443

    Purpose

    Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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