We narrowed to 35,947 results for: INO

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pColdI-GST-mGTSF2

Plasmid

#215714

Purpose

Bacterial expression of GST-tagged GTSF2

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF2 (Gtsf2 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-FRB-Rab5

Plasmid

#214272

Purpose

Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide2 in pX458

Plasmid

#211536

Purpose

sgRNA-2 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide1 in pX458

Plasmid

#211535

Purpose

sgRNA-1 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(T31N)-HA

Plasmid

#206401

Purpose

Mammalian expression of hARFRP1(T31N)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(WT)-HA

Plasmid

#206399

Purpose

Mammalian expression of hARFRP1

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(Q79L)-HA

Plasmid

#206400

Purpose

Mammalian expression of hARFRP1(Q79L)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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MLKS2del4exp

Plasmid

#130861

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of terminal four amino acids

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHcRed-CLCa

Plasmid

#196911

Purpose

visualization of clathrin-coated vesicle in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

Clathrin-Coated light chain

Tags

HcRed

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pOAD eIF4G1 (1-341)

Plasmid

#196833

Purpose

Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

eIF4G1 (TIF4631 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

amino acids 1-341

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-Miro1(∆TM)-SspB

Plasmid

#194007

Purpose

Optogenetic tool

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K301A

Plasmid

#195611

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

A822G, A823C, A824T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His

Plasmid

#195610

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

Promoter

T7

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-VC155

Plasmid

#194046

Purpose

Transient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Tags

AtOEP7 1–50aa

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-VN154

Plasmid

#194045

Purpose

Transient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VN154 (mVenus β-strands 1 to 7, amino acids 1−154)

Tags

AtOEP7 1–50aa

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Tesmin-BioID2-3xFLAG

Plasmid

#186815

Purpose

CAG promoter-driven expression of TESMIN-BioID2-3xFLAG

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Insert

Tesmin (Tesmin Mouse)

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pClgn-Tesmin-BioID2-3xFLAG

Plasmid

#186816

Purpose

Mouse Clgn promoter-driven expression of TESMIN-BioID2-3xFLAG

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Insert

Tesmin (Tesmin Mouse)

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

mouse Clgn promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pClgn-Tesmin-TurboID-3xFLAG

Plasmid

#186817

Purpose

Mouse Clgn promoter-driven expression of TESMIN-TurboID-3xFLAG

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Insert

Tesmin (Tesmin Mouse)

Tags

TurboID-3xFLAG

Expression

Mammalian

Promoter

mouse Clgn promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-24xopto-TetO-no01

Plasmid

#174887

Purpose

Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

24xopto-TetO

Use

Southern blot probe

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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