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pLARRYv2-T-Sapphire

Plasmid

#233209

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with T-Sapphire marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pNDL-60

Plasmid

#161720

Purpose

In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

Depositor

Adam Cohen

Article

McNamara et al Development. 2025 May 1;152(9):dev204506

Insert

cytosolic acvr2b-CIB1N (acvr2b Zebrafish)

Use

In vitro transcription

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iPASnFR-L

Plasmid

#225238

Purpose

Bacterial expression of iPASnFR-L

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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PB-LacI-eGFP

Plasmid

#213021

Purpose

piggyBac donor vector containing LacI-eGFP for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

LacI**-eGFP

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

eGFP

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMT445

Plasmid

#139386

Purpose

aTC inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

aTC inducible Nanoluc expression

Use

Synthetic Biology

Promoter

aTC inducible promoter

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAK_DR30_EF1a_CasRx_Hygro

Plasmid

#134841

Purpose

Lentiviral all in one expression of CasRx and guideRNA

Depositor

Michael Yaffe

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Tags

T2A Hygromycin

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_Efla_FLEX_APEX2-eGFPf

Plasmid

#171939

Purpose

Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein

Depositor

David Berson

Insert

APEX2-eGFPf

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Ef1a

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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AL13Rb

Plasmid

#161006

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Tags

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2834

Plasmid

#160673

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pVL1392 TMEM97

Plasmid

#157748

Purpose

Baculovirus transfer vectors to create a virus expressing human TMEM97

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Tags

flag

Expression

Insect

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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epi-ABEmax-NG

Plasmid

#135976

Purpose

Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
pET51b-NAD-cpLuc2

Plasmid

#135629

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc2 sensor

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK614

Plasmid

#110608

Purpose

BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

dCas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1221

Plasmid

#119260

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-hcvs-DHFR

Plasmid

#116044

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-hcvs-DHFR

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.718

Plasmid

#108519

Purpose

PBetI-YFP Reporter. Cho sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBetI-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.1459

Plasmid

#108521

Purpose

P3B5C-YFP Reporter. DHBA sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

P3B5C-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRYG

Plasmid

#113642

Purpose

Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

mRFP1-linker-sfGFP

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR6k-con-GFPuv

Plasmid

#106387

Purpose

Constitutive LlacO-1 promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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