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pESC-TYR

Plasmid

#222484

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and TYR1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-THR

Plasmid

#222479

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and THR1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-ADE

Plasmid

#222480

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and ADE2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-ARG

Plasmid

#222481

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and ARG1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCG-Oct-1

Plasmid

#53306

Purpose

plasmid contains POU2F1 (Oct-1) gene

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

Oct-1 (POU2F1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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OmpT

Plasmid

#213137

Purpose

OmpT_native membrane expression

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

OmpT

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Elk1 AdenoX

Plasmid

#121098

Purpose

Generation of adenovirus expressing full length ELK1

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Use

Adenoviral

Tags

HA

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pTet-ctl

Plasmid

#136693

Purpose

Control for pTet-Gli2shR

Depositor

LuZhe Sun

Article

Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. d

Type

Empty backbone

Use

Lentiviral

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS rev

Plasmid

#101327

Purpose

Encodes codon optimized NL4-3 rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-12S-E1A-HA-puro

Plasmid

#212671

Purpose

Express adenovirus E1A

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

12S-E1A

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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mV-VfAU1-LOV_226

Plasmid

#58686

Purpose

Expresses mVenus-fusion protein of VfAU1-LOV domain (Vaucheria frigida Aureochrome 1)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

mV-VfAU1-LOV

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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ins-cre_cryaa-Venus

Plasmid

#24333

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

Cre (cre )

Use

Cre/Lox

Tags

Venus

Expression

Worm

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCabp5-DsRed

Plasmid

#11157

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

Cabp5 promoter (Cabp5 Mouse)

Tags

DsRed2

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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TBRE-TK-Luc-MUT

Plasmid

#36245

Depositor

Bruce Blumberg

Article

Janesick et al Development. 2012 Mar;139(6):1213-24.

Insert

Two copies of the Brachyury T-box site

Tags

Luciferase

Expression

Mammalian

Mutation

Mutations in the Brachyury palindromic element:CA…

Promoter

TK minimal promoter

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSuper-gmiR-1

Plasmid

#171553

Purpose

a human miR-1-expressing plasmid

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

miR-1-2 (MIR1-2 Human)

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 Ub-CRT

Plasmid

#69619

Purpose

Express cytoplasmic mature CRT.

Depositor

Marta Hallak

Article

Goitea et al J Biol Chem. 2015 Jun 26;290(26):16403-1

Insert

Ubiquitina-Calreticulin (mature)

Tags

EGFP and Ubiquitin

Expression

Mammalian

Mutation

Deletion of signal peptide of calreticulin at the…

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCJH021_SC101_pLAC_Cas12c2_Kan

Plasmid

#183072

Purpose

Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c2

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSuperRetro-EFP siRNA2

Plasmid

#12450

Depositor

Dong-Er Zhang

Article

Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

Insert

siRNA against EFP (TRIM25 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only