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PcDNA-DEST47-CypB-Golgi C-term GFP

Plasmid

#36128

Depositor

Patrick Van Oostveldt

Publication

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
ZE38 pCS2P+ sp5l ORF

Plasmid

#17079

Depositor

Randall Moon

Publication

Thorpe et al Development. 2005 Apr. 132(8):1763-72.

Insert

sp5l ORF (sp5l Zebrafish)

Use

Zebrafish expression

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pAGM9121

Plasmid

#51833

Purpose

level 0 Universal acceptor

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

March 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pClneoFH human RASSF1A

Plasmid

#37016

Depositor

Yutaka Hata

Publication

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

rassf1A (RASSF1 Human)

Tags

FLAG and His

Expression

Mammalian

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-CFP-SaCas9-HF (without sgRNA)

Plasmid

#134470

Purpose

Human expression vector for SaCas9-HF variant.

Depositor

Zongli Zheng

Publication

Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

Insert

Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity

Use

CRISPR

Tags

SV40NLS-3xFLAG

Expression

Mammalian

Mutation

R245A, N413A, N419A, R654A

Promoter

CAG

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSpCas9(BB)-2A-mCherry

Plasmid

#197420

Purpose

Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

Depositor

Torsten Wittmann

Publication

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

Cas9

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-EGFP-ccdB

Plasmid

#14322

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAG303GPD-ccdB

Plasmid

#14134

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-YHAM-sgRNA_v7

Plasmid

#208356

Purpose

Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Publication

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-YHAM

Expression

Mammalian

Mutation

F48Y/S188H/V232A/E316M

Promoter

CMV

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Publication

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iFlpV

Plasmid

#140137

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

Depositor

Hongkui Zeng

Publication

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has service

AAV PHP.eB and AAV1

Insert

iFlpV

Use

AAV

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pICH47772

Plasmid

#48004

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pENTR221-FGFR4

Plasmid

#60531

Purpose

Entry vector for human FGFR4

Depositor

John Brognard

Publication

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

FGFR4 (FGFR4 Human)

Use

Gateway Cloning

Mutation

c2134a P712T

Promoter

NA

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pGWB435

Plasmid

#74825

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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cytosolic moPrP (3F4)

Plasmid

#1321

Depositor

Susan Lindquist

Publication

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

N-term truncation (to make gene cytosolic), vario…

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GST HP1 beta

Plasmid

#24075

Depositor

Naoko Tanese

Publication

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 beta (CBX1 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
CMV-LgBit

Plasmid

#234738

Purpose

Mammalian expression of large NanoBit fragment (LgBit)

Depositor

Nevin Lambert

Publication

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

LgBit

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

Plasmid

#189752

Purpose

Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR(T/A)

Plasmid

#192447

Purpose

Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

Depositor

Jin Zhang

Publication

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Thr 6 in AMPK substrate mutated to Ala.

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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JNK-KTR-mStrawberry reporter

Plasmid

#158689

Purpose

This plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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