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pPOTv7-blast-mScarlet

Plasmid

#179818

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mNG

Plasmid

#179821

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-hSeparase-wt

Plasmid

#33016

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pI4-MTU-DO-URA

Plasmid

#160025

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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TRPML3-CFP

Plasmid

#18834

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

CFP

Expression

Mammalian

Mutation

Q165R, F210Y

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCfB4786

Plasmid

#106142

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2_164

Plasmid

#199096

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI plus NGS barcode 4, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_165

Plasmid

#199097

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSC101ts ORI plus NGS barcode 10, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_161

Plasmid

#199093

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAL5000 ORI plus NGS barcode 1, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_162

Plasmid

#199094

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI plus NGS barcode 2, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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hGFAP-Roxed-Cre

Plasmid

#51263

Purpose

Expression of the Dre-dependent Roxed-Cre gene under the control of the human GFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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LI A-B EF1 c2

Plasmid

#54435

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

promoter region of A. thaliana EF1alpha1 /AT1G07940.1

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mVenus

Plasmid

#154898

Purpose

mVenus entry vector

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Type

Empty backbone

Use

Entry vector

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Plasmid

#159797

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-luxAB-ssrAec

Plasmid

#27062

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB-ssrAec

Expression

Bacterial

Available Since

July 6, 2011

Availability

Academic Institutions and Nonprofits only

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LI F-G Kan

Plasmid

#104523

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Kanamycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-AtAEP2

Plasmid

#159529

Purpose

Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Arabidopsis thaliana asparaginyl endopeptidase 2

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarletI

Plasmid

#201442

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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hPRL3_1-169

Plasmid

#209796

Purpose

Bacterial expression of human PRL-3 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2004 Mar 19. 279(12):11882-

Insert

PRL-3 (PTP4A3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-17

Plasmid

#119996

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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