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pBbB19k-GFP

Plasmid

#126135

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pWRS_1001

Plasmid

#192205

Purpose

lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

Depositor

William Sellers

Article

Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and RNAi

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-HALOnls

Plasmid

#121937

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

MCP-HALOnls

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSELIS-RamR

Plasmid

#202631

Purpose

Enables dual growth and fluorescence based enrichment of RamR biosensors

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

Sh ble

Use

Synthetic Biology

Expression

Bacterial

Promoter

Lambda promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGY199

Plasmid

#239801

Purpose

Express EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

EGFP

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBbB15a-GFP

Plasmid

#126130

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)

Plasmid

#70210

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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5xGAL4-TATA-luciferase

Plasmid

#46756

Purpose

Luciferase reporter gene for factors with GAL4 DNA binding domains

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Luciferase

Use

Luciferase

Promoter

minimal TATA

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pM201

Plasmid

#173179

Purpose

dCas9-24xGCN4

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9-24xGCN4

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-MP2

Plasmid

#36097

Purpose

3rd generation lentiviral plasmid

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pWRS3001_VCR1_WCR3

Plasmid

#192206

Purpose

VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone

Depositor

William Sellers

Article

Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

Insert

VCR1-WCR3 tracrRNA

Expression

Mammalian

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-325

Plasmid

#195450

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF1 BS - minTK - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND1-1397C-AD

Plasmid

#183892

Purpose

Expresses TALED_Left-ND1-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-ND1-1397N

Plasmid

#183898

Purpose

Expresses TALED_Right-ND1-1397N in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND1-AD-E1347A

Plasmid

#183894

Purpose

Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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YSBE 610 : pHYG_6xHA

Plasmid

#92091

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pC007 - pRFP constitutive expression

Plasmid

#79156

Purpose

RFP constitutively expressed

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

RFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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YSBE 557 : pNAT_GFP

Plasmid

#92085

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, NAT selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBbA19k-RFP

Plasmid

#126105

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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