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Showing: 7501 - 7520 of 11375 results

  7. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  8. pMT449

    Plasmid
    #139389
    Purpose
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
    Use
    Synthetic Biology
    Promoter
    Bile Acid inducible Promoter
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139389-plasmid-map-sequence-id-313839

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  16. pSLQ10531

    Plasmid
    #239267
    Purpose
    Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NLS-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239267-plasmid-map-sequence-id-475551

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  17. XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

    Plasmid
    #233386
    Purpose
    Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    IL2Rb(ecto+TM)-ADAR2dd
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233386-plasmid-map-sequence-id-466377

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Showing: 7501 - 7520 of 11375 results