We narrowed to 10,081 results for: Gnas

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FLAG-3C-sGFP11_pcDNA3.1(-)

Plasmid

#182932

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 11, FLAG tag, and HA signa…

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Promoter

pMT

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pSR58.6

Plasmid

#63176

Purpose

Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-H2-18 Light chain

Plasmid

#216308

Purpose

Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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HiBiT-GLP1R Fusion Vector

Plasmid

#238559

Purpose

Express HiBiT-GLP1R Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

GLP1R (GLP1R Human)

Tags

HiBiT

Expression

Mammalian

Mutation

Domain 24-463 (native signal peptide deleted)

Promoter

CMV

Available Since

Oct. 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pYHY2

Plasmid

#216108

Purpose

Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Insert

NanoLuc

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Tags

3xFLAG tag and BT0294 signal peptide

Expression

Bacterial

Promoter

BfP1E6 promoter

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA63_HelperΔgIII

Plasmid

#190433

Purpose

M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 genes I-V and VII-XI

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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Dazl-CPG-TV

Plasmid

#70144

Purpose

a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL

Use

Mouse Targeting and Synthetic Biology

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA63_Helper

Plasmid

#190432

Purpose

M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 genes: I-XI

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PB-GFP1-10-HygR

Plasmid

#223675

Purpose

To check the split GFP signals in mitochondria (1)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP1-10

Expression

Mammalian

Promoter

cmv

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+Lux

Plasmid

#26159

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3-mRuby3

Plasmid

#203492

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pIBA2-Int-SpyTag

Plasmid

#198038

Purpose

Expression plasmid for producing SpyTagged stalled secretion variant of intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

lacks native signal peptide (amino acids 1-39)

Promoter

tet

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ssSBP-EGFP

Plasmid

#65277

Purpose

to synchronize transport of secretory soluble GFP from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

EGFP

Tags

IL-2 signal sequence and Streptavidin Binding Pr…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a MDHP_YEAST

Plasmid

#204233

Purpose

Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDHP_YEAST (MDH3 Budding Yeast)

Tags

6X His

Expression

Bacterial

Mutation

The last 3 C-terminal residues (SKL) are the pero…

Promoter

Lac

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mTagBFP-KDEL

Plasmid

#87163

Purpose

Fluorescent marker of endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

mTagBFP-KDEL

Tags

Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

Expression

Mammalian

Promoter

EF-1 alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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