We narrowed to 21,831 results for: SPR

Showing: 7521 - 7540 of 21831 results

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pLenti-LmoCas8b2

Plasmid

#126488

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas7

Plasmid

#126489

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas5

Plasmid

#126490

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6

Plasmid

#126491

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1825_pCR8-dCasRx-RBM38

Plasmid

#118662

Purpose

Gateway Donor; dCasRx-RBM38 ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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hgRNA-A51_pLKO-Hyg

Plasmid

#100567

Purpose

hgRNA-A51 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A51

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-G21_pLKO-Hyg

Plasmid

#100565

Purpose

hgRNA-G21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-G21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-E21_pLKO-Hyg

Plasmid

#100563

Purpose

hgRNA-E21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-E21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW:U6

Plasmid

#85981

Purpose

Golden Gate Level 0 cassette encoding the U6 promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

T. pseudonana U6 promoter

Use

CRISPR; Diatom (t. pseudonana)

Promoter

none

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGY53

Plasmid

#221709

Purpose

Empty Cas12a vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Zeo

Plasmid

#227271

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Blast

Plasmid

#227260

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas8b2

Plasmid

#126482

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas7

Plasmid

#126483

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas5

Plasmid

#126484

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T2_AmpR

Plasmid

#119159

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T3_AmpR

Plasmid

#119160

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T1_AmpR

Plasmid

#119158

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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